Tetracycline-inducible gene expression system in Leishmania mexicana

被引：30
|
作者：
Kraeva, Natalya [1 ]
Ishemgulova, Aygul [1 ]
Lukes, Julius [2 ,3 ,4 ]
Yurchenko, Vyacheslav [1 ,2 ]
机构：
[1] Univ Ostrava, Fac Sci, Life Sci Res Ctr, Ostrava 71000, Czech Republic
[2] Czech Acad Sci, Ctr Biol, Inst Parasitol, Ceske Budejovice 37005, Budweis, Czech Republic
[3] Univ South Bohemia, Fac Sci, Ceske Budejovice 37005, Budweis, Czech Republic
[4] Canadian Inst Adv Res, Toronto, ON M5G 1Z8, Canada
来源：
MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 2014年 / 198卷 / 01期
基金：
欧盟第七框架计划;
关键词：
Leishmania mexicana; Gene expression; Tet-inducible system;
D O I：
10.1016/j.molbiopara.2014.11.002
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Here we present a T7-driven, tetracycline-inducible system for protein expression in human pathogen Leishmania mexicana. The gene expression in this strain is tightly regulated and dose- and timedependent. This system can be widely used by the parasitology community to analyze effects of genes of interest on biology, physiology and virulence of parasites causing cutaneous leishmaniases. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：11 / 13
页数：3
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