Effect of microbial community structure in inoculum on the stimulation of direct interspecies electron transfer for methanogenesis

被引：24

作者：

Kang, Hyun-Jin ^{[1
]}

Lee, Sang-Hoon ^{[1
]}

Lim, Tae-Guen ^{[1
]}

Park, Hee-Deung ^{[1
,2
]}

机构：

[1] Korea Univ, Sch Civil Environm & Architectural Engn, Seoul 02841, South Korea

[2] Korea Univ, KU KIST Grad Sch Converging Sci & Technol, Seoul 02841, South Korea

来源：

BIORESOURCE TECHNOLOGY | 2021年 / 332卷

基金：

新加坡国家研究基金会;

关键词：

Direct interspecies electron transfer (DIET); Inoculum; Methanogenesis; Methanogenic performance; Microbial community structure; GRANULAR ACTIVATED CARBON; ANAEROBIC-DIGESTION; SYNTROPHIC METABOLISM; METHANE PRODUCTION; ENHANCEMENT; PROPIONATE; BUTYRATE; BIOCHAR; PROMOTE; BIOMASS;

D O I：

10.1016/j.biortech.2021.125100

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

To investigate how the seed microbial community structure affects the improvement of methanogenesis efficiency through direct interspecies electron transfer (DIET), a biomethane potential (BMP) test was conducted using sludge collected from a total of six anaerobic digesters. DIET-stimulating microbial populations were investigated by 16S rRNA gene sequence analysis. Correlations between microbial community composition and methane production performance by DIET were analyzed. The methane production rate increased under all conditions when granular activated carbon (GAC) was injected regardless of the inoculum type. However, redundancy analysis indicated a significant correlation between the inoculum microbial community and lag time. In a network analysis, Methanolinea species distributed in the inocula formed a single modularity with lag time, suggesting that the methanogens in the inocula might reduce the lag time of methanogenesis through DIET. Overall, this study revealed that the inoculum microbial community composition is an important factor affecting methane production efficiency by DIET.

引用

页数：10

共 36 条

[1] Effect of inoculum on the anaerobic digestion of food waste accounting for the concentration of trace elements [J].

Alexis Parra-Orobio, Brayan ;

Donoso-Bravo, Andres ;

Camilo Ruiz-Sanchez, Juan ;

Jimena Valencia-Molina, Karen ;

Torres-Lozada, Patricia .

WASTE MANAGEMENT, 2018, 71 :342-349

[2]

Bastian M., 2009, P INT AAAI C WEB SOC, P361, DOI DOI 10.1609/ICWSM.V3I1.13937

[3] QIIME allows analysis of high-throughput community sequencing data [J].

Caporaso, J. Gregory ;

Kuczynski, Justin ;

Stombaugh, Jesse ;

Bittinger, Kyle ;

Bushman, Frederic D. ;

Costello, Elizabeth K. ;

Fierer, Noah ;

Pena, Antonio Gonzalez ;

Goodrich, Julia K. ;

Gordon, Jeffrey I. ;

Huttley, Gavin A. ;

Kelley, Scott T. ;

Knights, Dan ;

Koenig, Jeremy E. ;

Ley, Ruth E. ;

Lozupone, Catherine A. ;

McDonald, Daniel ;

Muegge, Brian D. ;

Pirrung, Meg ;

Reeder, Jens ;

Sevinsky, Joel R. ;

Tumbaugh, Peter J. ;

Walters, William A. ;

Widmann, Jeremy ;

Yatsunenko, Tanya ;

Zaneveld, Jesse ;

Knight, Rob .

NATURE METHODS, 2010, 7 (05) :335-336

[4] The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: A review [J].

Demirel B. ;

Scherer P. .

Reviews in Environmental Science and Bio/Technology, 2008, 7 (2) :173-190

[5]

Edgar RC, 2013, NAT METHODS, V10, P996, DOI [10.1038/NMETH.2604, 10.1038/nmeth.2604]

[6] Effect of inoculum sources on the anaerobic digestion of rice straw [J].

Gu, Yu ;

Chen, Xiaohua ;

Liu, Zhanguang ;

Zhou, Xuefei ;

Zhang, Yalei .

BIORESOURCE TECHNOLOGY, 2014, 158 :149-155

[7] RNA-based spatial community analysis revealed intra-reactor variation and expanded collection of direct interspecies electron transfer microorganisms in anaerobic digestion [J].

Guo, Bing ;

Zhang, Yingdi ;

Zhang, Lei ;

Zhou, Yun ;

Liu, Yang .

BIORESOURCE TECHNOLOGY, 2020, 298

[8] Syntrophic anaerobic photosynthesis via direct interspecies electron transfer [J].

Ha, Phuc T. ;

Lindemann, Stephen R. ;

Shi, Liang ;

Dohnalkova, Alice C. ;

Fredrickson, James K. ;

Madigan, Michael T. ;

Beyenal, Haluk .

NATURE COMMUNICATIONS, 2017, 8

[9] Reactor performances and microbial communities of biogas reactors: effects of inoculum sources [J].

Han, Sheng ;

Liu, Yafeng ;

Zhang, Shicheng ;

Luo, Gang .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 100 (02) :987-995

[10] Recent advances in methanogenesis through direct interspecies electron transfer via conductive materials: A molecular microbiological perspective [J].

Kang, Hyun-Jin ;

Lee, Sang-Hoon ;

Lim, Tae-Guen ;

Park, Jeong-Hoon ;

Kim, Boram ;

Buffiere, Pierre ;

Park, Hee-Deung .

BIORESOURCE TECHNOLOGY, 2021, 322

← 1 2 3 4 →