Raft Polymerization of N,N-Dimethylacrylamide from Magnetic Poly(2-hydroxyethyl methacrylate) Microspheres to Suppress Nonspecific Protein Adsorption

被引:12
作者
Cao, Xueteng [1 ]
Horak, Daniel [2 ]
An, Zesheng [1 ]
Plichta, Zdenek [2 ]
机构
[1] Shanghai Univ, Coll Environm Sci & Chem Engn, Inst Nanochem & Nanobiol, Shanghai 200444, Peoples R China
[2] Acad Sci Czech Republ, Inst Macromol Chem, Prague 16206 6, Czech Republic
关键词
magnetic polymers; microspheres; N; N-dimethylacrylamide; proteins; reversible addition fragmentation chain transfer (RAFT); POLY(GLYCIDYL METHACRYLATE); PARTICLES; CLICK; BRUSHES; CARBOHYDRATE; PURIFICATION; FABRICATION; LATEX; TOOL; DNA;
D O I
10.1002/pola.27939
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Nonspecific interaction is a key parameter affecting the efficiency of proteins, nucleic acids or cell separation. Currently, many approaches to introduce antifouling properties to materials have been developed. Among these, surface modification with polymer brushes plays a prominent role. The aim of this study was to synthesize new magnetic microspheres grafted with poly(N,N-dimethylacrylamide) (PDMA) that resist nonspecific protein adsorption. Monodisperse macroporous poly(2-hydroxyethyl methacrylate) (PHEMA) microspheres, 4 mu m in size, were synthesized by a multiple swelling polymerization method. To render the microspheres magnetic, iron oxide was precipitated inside the microsphere pores. Functional carboxyl groups, introduced by the hydrolysis of the 2-(methacryloyl)-oxyethyl acetate (HEMA-Ac) comonomer, were used to react with propargylamine, followed by coupling of a chain transfer agent via an azide-alkyne click reaction. PDMA was grafted from the PHEMA microspheres using reversible addition-fragmentation chain transfer polymerization (RAFT), resulting in surfaces with more than 81 wt % PDMA attached. The successful modification of the microspheres was confirmed by XPS. The magnetic microspheres grafted with PDMA showed excellent antifouling properties as tested in bovine serum protein solutions. (C) 2015 Wiley Periodicals, Inc.
引用
收藏
页码:1036 / 1043
页数:8
相关论文
共 47 条
[1]   Polymer brushes by anionic and cationic Surface-Initiated Polymerization (SIP) [J].
Advincula, Rigoberto .
SURFACE-INITIATED POLYMERIZATION I, 2006, 197 (01) :107-136
[2]   Heterofunctional polymers and core-shell nanoparticles via cascade aminolysis/Michael addition and alkyne-azide click reaction of RAFT polymers [J].
An, Zesheng ;
Tang, Wei ;
Wu, Minghong ;
Jiao, Zheng ;
Stucky, Galen D. .
CHEMICAL COMMUNICATIONS, 2008, (48) :6501-6503
[3]   Polymer Brushes via Surface-Initiated Controlled Radical Polymerization: Synthesis, Characterization, Properties, and Applications [J].
Barbey, Raphael ;
Lavanant, Laurent ;
Paripovic, Dusko ;
Schuewer, Nicolas ;
Sugnaux, Caroline ;
Tugulu, Stefano ;
Klok, Harm-Anton .
CHEMICAL REVIEWS, 2009, 109 (11) :5437-5527
[4]   'Click' chemistry in polymer and materials science [J].
Binder, Wolfgang H. ;
Sachsenhofer, Robert .
MACROMOLECULAR RAPID COMMUNICATIONS, 2007, 28 (01) :15-54
[5]   Microwave assisted "click" chemistry for the synthesis of multiple labeled-carbohydrate oligonucleotides on solid support [J].
Bouillon, Camille ;
Meyer, Albert ;
Vidal, Sebastien ;
Jochum, Anne ;
Chevolot, Yann ;
Cloarec, Jean-Pierre ;
Praly, Jean-Pierre ;
Vasseur, Jean-Jacques ;
Morvan, Francois .
JOURNAL OF ORGANIC CHEMISTRY, 2006, 71 (12) :4700-4702
[6]   Magnetic resonance imaging of functional Schwann cell transplants labelled with magnetic microspheres [J].
Dunning, Mark D. ;
Kettunen, Mikko I. ;
Constant, Charles Ffrench ;
Franklin, Robin J. M. ;
Brindle, Kevin M. .
NEUROIMAGE, 2006, 31 (01) :172-180
[7]   Reactive magnetic poly(divinylbenzene-co-glycidyl methacrylate) colloidal particles for specific antigen detection using microcontact printing technique [J].
Eissa, Mohamed M. ;
Rahman, Md. Mahbubor ;
Zine, Nadia ;
Jaffrezic, Nicole ;
Errachid, Abdelhamid ;
Fessi, Hatem ;
Elaissari, Abdelhamid .
ACTA BIOMATERIALIA, 2013, 9 (03) :5573-5582
[8]   Hydrophilic magnetic latex for nucleic acid extraction, purification and concentration [J].
Elaïssari, A ;
Rodrigue, M ;
Meunier, F ;
Herve, C .
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 2001, 225 (1-2) :127-133
[9]   Non-biofouling materials prepared by atom transfer radical polymerization grafting of 2-methacryloloxyethyl phosphorylcholine: Separate effects of graft density and chain length on protein repulsion [J].
Feng, W ;
Brash, JL ;
Zhu, SP .
BIOMATERIALS, 2006, 27 (06) :847-855
[10]   Fabrication of Unique Chemical Patterns and Concentration Gradients with Visible Light [J].
Fors, Brett P. ;
Poelma, Justin E. ;
Menyo, Matthew S. ;
Robb, Maxwell J. ;
Spokoyny, Daniel M. ;
Kramer, John W. ;
Waite, J. Herbert ;
Hawker, Craig J. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2013, 135 (38) :14106-14109