Silica immobilization of an enzyme through genetic engineering of the diatom Thalassiosira pseudonana

被引：90
作者：
Poulsen, Nicole
Berne, Cecile
Spain, Jim
Kroeger, Nils
机构：
[1] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA
[2] USAF, Res Lab, Tyndall AFB, FL 32403 USA
[3] Georgia Inst Technol, Sch Civil & Environm Engn, Atlanta, GA 30332 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 11期
关键词：
diatoms; enzyme catalysis; gene technology; immobilization; silica;
D O I：
10.1002/anie.200603928
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Chemical Equation Presented) Biosilica structures containing foreign proteins with desired functionalities can be generated by a molecular genetic method. An enzyme immobilized in diatom silica was found to display better activity than purified hydroxylaminobenzene mutase in solution. Such immobilized active biomolecules may find application for the fabrication of sensor materials and reusable catalysts. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
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收藏
页码：1843 / 1846
页数：4
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