Regulation of lipoxygenase-1 and Dectin-1 on interleukin-10 in mouse Aspergillus fumigatus keratitis

被引：8

作者：

Che, Cheng-Ye ^{[1
]}

Yuan, Ke-Lan ^{[1
]}

Zhao, Gui-Qiu ^{[1
]}

Li, Cui ^{[1
]}

Lin, Jing ^{[1
]}

Zhu, Guo-Qiang ^{[1
]}

Liu, Min ^{[1
]}

机构：

[1] Qingdao Univ, Dept Ophthalmol, Affiliated Hosp, 16 Jiangsu Rd, Qingdao 266003, Shandong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF OPHTHALMOLOGY | 2018年 / 11卷 / 06期

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

keratitis; Aspergillus fumigatus; Dectin-1; lipoxygenase-1; interleukin-10; INNATE IMMUNITY; PROTECTIVE IMMUNITY; CYTOKINE SYNTHESIS; CANDIDA-ALBICANS; DENDRITIC CELLS; IL-10; RECOGNITION; LOX-1; RECEPTORS; INDUCTION;

D O I：

10.18240/ijo.2018.06.02

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

AIM: To investigate the regulation of lipoxygenase (LOX)-1 and Dectin-1 on interleukin-10 (IL-10) production in mice with Aspergillus fumigatus (A. fumigatus) keratitis. METHODS: The corneas of C57BL/6 mice were pretreated with LOX-1 inhibitor Poly(I) or Dectin-1 siRNA separately before the infection of A. fumigatus. Polymerase chain reaction (PCR) and Western blot were used to detect the expression of IL-10. RESULTS: The mRNA and protein expressions of IL-10 were significantly increased in mice with A. fumigatus keratitis. Compared with the group pretreated with sterile water before infection, Poly(I) pretreatment suppressed IL-10 expression significantly. Compared with the group pretreated with scrambled siRNA before infection, Dectin-1 siRNA pretreatment significantly reduced IL-10 expression in response to A. fumigatus infection. CONCLUSION: LOX-1 and Dectin-1 regulate IL-10 production in mouse A. fumigatus keratitis.

引用

页码：905 / 909

页数：5

共 43 条

[1] Effect of IL-10 on LOX-1 expression, signalling and functional activity: An atheroprotective response [J].

Arjuman, Albina ;

Chandra, Nimai C. .

DIABETES & VASCULAR DISEASE RESEARCH, 2013, 10 (05) :442-451

[2]

Brown GD, 2006, NAT REV IMMUNOL, V6, P33, DOI 10.1038/nri1745

[3] Immune recognition -: A new receptor for β-glucans [J].

Brown, GD ;

Gordon, S .

NATURE, 2001, 413 (6851) :36-37

[4] Dual function of C-type lectin-like receptors in the immune system [J].

Cambi, A ;

Figdor, CG .

CURRENT OPINION IN CELL BIOLOGY, 2003, 15 (05) :539-546

[5] Yeast zymosan, a stimulus for TLR2 and dectin-1, induces regulatory antigen-presenting cells and immunological tolerance [J].

Dillon, S ;

Agrawal, S ;

Banerjee, K ;

Letterio, J ;

Denning, TL ;

Oswald-Richter, K ;

Kasprowicz, DJ ;

Kellar, K ;

Pare, J ;

van Dyke, T ;

Ziegler, S ;

Unutmaz, D ;

Pulendran, B .

JOURNAL OF CLINICAL INVESTIGATION, 2006, 116 (04) :916-928

[6] Dectin-1 Regulates IL-10 Production via a MSK1/2 and CREB Dependent Pathway and Promotes the Induction of Regulatory Macrophage Markers [J].

Elcombe, Suzanne E. ;

Naqvi, Shaista ;

Van den Bosch, Mirjam W. M. ;

MacKenzie, Kirsty F. ;

Cianfanelli, Francesca ;

Brown, Gordon D. ;

Arthur, J. Simon C. .

PLOS ONE, 2013, 8 (03)

[7] Medical interventions for fungal keratitis [J].

FlorCruz, N., V ;

Peczon, Jr, I .

COCHRANE DATABASE OF SYSTEMATIC REVIEWS, 2008, (01)

[8] Dectin-1 mediates macrophage recognition of Candida albicans yeast but not filaments [J].

Gantner, BN ;

Simmons, RM ;

Underhill, DM .

EMBO JOURNAL, 2005, 24 (06) :1277-1286

[9] LOX-1 and TLR4 affect each other and regulate the generation of ROS in A. fumigatus keratitis [J].

Gao, Xinran ;

Zhao, Guiqiu ;

Li, Cui ;

Lin, Jing ;

Jiang, Nan ;

Wang, Qian ;

Hu, Liting ;

Xu, Qiang ;

Peng, Xudong ;

He, Kun ;

Zhu, Guoqiang .

INTERNATIONAL IMMUNOPHARMACOLOGY, 2016, 40 :392-399

[10] Pattern recognition receptors: Doubling up for the innate immune response [J].

Gordon, S .

CELL, 2002, 111 (07) :927-930

← 1 2 3 4 5 →