High-frequency 263 GHz PELDOR

被引：8
作者：
Tkach, Igor [1 ]
Halbmair, Karin [1 ]
Hoebartner, Claudia [2 ,3 ]
Bennati, Marina [1 ,3 ]
机构：
[1] Max Planck Inst Biophys Chem, Res Grp EPR Spect, D-37077 Gottingen, Germany
[2] Max Planck Inst Biophys Chem, Res Grp Nucle Acid Chem, D-37077 Gottingen, Germany
[3] Univ Gottingen, Inst Organ & Biomol Chem, D-37073 Gottingen, Germany
来源：
APPLIED MAGNETIC RESONANCE | 2014年 / 45卷 / 10期
关键词：
ELECTRON DOUBLE-RESONANCE; DISTANCE MEASUREMENTS; NANOMETER-RANGE; SPIN LABELS; EPR; BIOMACROMOLECULES; ORIENTATION; DEER; SPECTROSCOPY; GEOMETRY;
D O I：
10.1007/s00723-014-0581-z
中图分类号：
O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];
学科分类号：
070203 ; 070304 ; 081704 ; 1406 ;
摘要：
Pulsed electron-electron double resonance (PELDOR/DEER) at high frequencies can provide information on the relative orientation of paramagnetic centres or spin labels, if those are rigidly oriented in a host biomolecule and experiments are performed with sufficient orientation selectivity. We present the first comparative PELDOR study at 263 and 94 GHz on a model RNA system containing rigid nitroxides. We show that at 263 GHz still considerable modulation depth is observed and orientation selectivity is significant, particularly in g (x)-g (y) plane of the nitroxides.
引用
收藏
页码：969 / 979
页数：11
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