Strong cellular and humoral immune responses induced by transcutaneous immunization with HBsAg DNA-cationic deformable liposome complex

被引：38

作者：

Wang, Jing ^{[1
]}

Hu, Jin-Hong ^{[1
]}

Li, Feng-Qian ^{[1
]}

Liu, Guan-Zhong ^{[1
]}

Zhu, Quan-Gang ^{[1
]}

Liu, Ji-Yong ^{[1
]}

Ma, Hong-Jun ^{[1
]}

Peng, Cheng ^{[1
]}

Si, Fu-Guo ^{[1
]}

机构：

[1] Second Mil Med Univ, Changhai Hosp, Dept Pharm, Shanghai 200433, Peoples R China

来源：

EXPERIMENTAL DERMATOLOGY | 2007年 / 16卷 / 09期

关键词：

cationic; deformable liposome; DNA octadecylamine; transcutaneous immune;

D O I：

10.1111/j.1600-0625.2007.00584.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Transcutaneous immunization presents a major challenge on account of poor permeability of antigens through the skin barrier. To overcome this limitation, the deformable liposome could be a better method for transcutaneous delivery of these antigens. In this study, hepatitis B surface antigen (HBsAg) plasmid DNA-cationic complex deformable liposome was utilized as a mode for enhanced immunity against the antigen. Deformable liposome was prepared by conventional rotary evaporation method and characterized for various parameters such as vesicles shape and surface morphology, size and size distribution, entrapment efficiency, elasticity and stability. The immune stimulating activity was studied by measuring serum anti-HBsAg titre and cytokines level (interleukin-4 and interferon-gamma) following topical application of liposome in BALB/c mice and results were compared with deformable liposome encapsulated DNA applied topically as well as naked DNA and pure recombinant HBsAg, administered intramuscularly. It was observed that deformable liposome elicited a comparable serum antibody titre and endogenous cytokines levels compared to other vaccinations. The study signifies the potential of deformable liposome as DNA vaccine carriers for effective transcutaneous immunization.

引用

页码：724 / 729

页数：6

共 18 条

[1] Intramuscular plasmid DNA electrotransfer biodistribution and degradation [J].

Bureau, MF ;

Naimi, S ;

Ibad, RT ;

Seguin, J ;

Georger, C ;

Arnould, E ;

Maton, L ;

Blanche, F ;

Delaere, P ;

Schennan, D .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2004, 1676 (02) :138-148

[2] Ultradeformable lipid vesicles can penetrate the skin and other semi-permeable barriers unfragmented.: Evidence from double label CLSM experiments and direct size measurements [J].

Cevc, G ;

Schätzlein, A ;

Richardsen, H .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2002, 1564 (01) :21-30

[3] Topical vaccination of DNA antigens: Topical delivery of DNA antigens [J].

Choi, MJ ;

Maibach, HI .

SKIN PHARMACOLOGY AND APPLIED SKIN PHYSIOLOGY, 2003, 16 (05) :271-282

[4]

Choi Myeong Jun, 2006, Current Drug Delivery, V3, P37, DOI 10.2174/156720106775197484

[5]

Cui Zhengrong, 2006, Current Drug Delivery, V3, P29, DOI 10.2174/156720106775197538

[6]

Dean Hansi J, 2005, Expert Opin Drug Deliv, V2, P227, DOI 10.1517/17425247.2.2.227

[7]

Foldvari Marianna, 2006, Current Drug Delivery, V3, P89, DOI 10.2174/156720106775197501

[8] Needle-free vaccine delivery [J].

Giudice, EL ;

Campbell, JD .

ADVANCED DRUG DELIVERY REVIEWS, 2006, 58 (01) :68-89

[9] DNA immunization - a new chance in vaccine research? [J].

Henke, A .

MEDICAL MICROBIOLOGY AND IMMUNOLOGY, 2002, 191 (3-4) :187-190

[10] Skin irritants and contact sensitizers induce Langerhans cell migration and maturation at irritant concentration [J].

Jacobs, JJL ;

Lehé, CL ;

Hasegawa, H ;

Elliott, GR ;

Das, PK .

EXPERIMENTAL DERMATOLOGY, 2006, 15 (06) :432-440

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