Modified protocols for rapid carrot genomic DNA extraction and AFLP™ analysis using silver stain or radioisotopes

被引：56
作者：
Briard, M
Le Clerc, V
Grzebelus, D
Senalik, D
Simon, PW
机构：
[1] Inst Natl Hort, Angers, France
[2] Krakow Agr Univ, Krakow, Poland
[3] Univ Wisconsin, USDA ARS, Madison, WI 53706 USA
来源：
PLANT MOLECULAR BIOLOGY REPORTER | 2000年 / 18卷 / 03期
关键词：
AFLP; DNA extraction; electrophoresis; molecular markers; polyacrylamide; radioactivity; silver staining;
D O I：
10.1007/BF02823994
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
AFLP(TM) is one of the most frequently used techniques for identification of molecular markers. We have modified the procedures for genomic DNA extraction, AFLP product generation and silver staining in order to speed up analyses and screen large numbers of plant samples. Using this protocol, we were able to achieve an 82% reduction of costs without compromising the reliability and quality of data gathered.
引用
收藏
页码：235 / 241
页数：7
相关论文
共 6 条
[1]   FAST AND SENSITIVE SILVER STAINING OF DNA IN POLYACRYLAMIDE GELS [J].
BASSAM, BJ ;
CAETANOANOLLES, G ;
GRESSHOFF, PM .
ANALYTICAL BIOCHEMISTRY, 1991, 196 (01) :80-83
[2]   Effects of plant tissue and DNA purification method on randomly amplified polymorphic DNA-based genetic fingerprinting analysis in carrot [J].
Boiteux, LS ;
Fonseca, MEN ;
Simon, PW .
JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 1999, 124 (01) :32-38
[3]   Silver staining and recovery of AFLP(TM) amplification products on large denaturing polyacrylamide gels [J].
Chalhoub, BA ;
Thibault, S ;
Laucou, V ;
Rameau, C ;
Hofte, H ;
Cousin, R .
BIOTECHNIQUES, 1997, 22 (02) :216-&
[4]  
Sambrook J., 2002, MOL CLONING LAB MANU
[5]   Linkage relationships among molecular markers and storage root traits of carrot (Daucus carota L. ssp sativus) [J].
Vivek, BS ;
Simon, PW .
THEORETICAL AND APPLIED GENETICS, 1999, 99 (1-2) :58-64
[6]   AFLP - A NEW TECHNIQUE FOR DNA-FINGERPRINTING [J].
VOS, P ;
HOGERS, R ;
BLEEKER, M ;
REIJANS, M ;
VANDELEE, T ;
HORNES, M ;
FRIJTERS, A ;
POT, J ;
PELEMAN, J ;
KUIPER, M ;
ZABEAU, M .
NUCLEIC ACIDS RESEARCH, 1995, 23 (21) :4407-4414
1