Mechanisms of Cr(VI) reduction by Bacillus sp. CRB-1, a novel Cr(VI)-reducing bacterium isolated from tannery activated sludge

被引:64
|
作者
Zhu, Yunfei [1 ]
Yan, Junwei [1 ]
Xia, Li [1 ]
Zhang, Xiang [1 ]
Luo, Lixin [1 ]
机构
[1] South China Univ Technol, Sch Biol & Biol Engn, Guangzhou 510006, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
Chromium; Bacillus; Cr(VI) reduction; Chromate reductase; Cr(III)-EPS complexes; SHEWANELLA-ONEIDENSIS MR-1; CHROMATE REDUCTION; HEXAVALENT CHROMIUM; RESISTANCE GENES; STRESS; BIOREMEDIATION; SALINITY; SITE; CHRA; EPS;
D O I
10.1016/j.ecoenv.2019.109792
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Cr(VI) reduction by microorganisms has been extensively reported, however, the mechanism of Cr(VI) reduction varies among different microorganisms. In this study, a Cr(VI)-reducing bacterium identified as Bacillus sp. was isolated from tannery activated sludge, strain CRB-1 was able to completely reduce 50 mg/L of Cr(VI) within 24 h under aerobic conditions and exhibited considerable Cr(VI) removal efficiency in the pH range from 7.0 to 9.0, temperature 24-42 degrees C. Cr(VI) reduction assays with resting cells, permeabilized cells, and subcellular fractions suggested that Cr(VI) reduction mainly occurred in the cytoplasm. According to qRT-PCR analysis, a chrA gene and a nitR2 gene were up-regulated under Cr(VI) stress. Heterologous expression of the chrA gene and the nitR2 gene indicated that ChrA was associated with Cr(VI) resistance, while NitR2 was responsible for Cr(VI) reduction. Furthermore, soluble end products were detected. On the basis of FTIR, it was speculated that the formation of soluble end products may be due to the complexation of EPS with Cr(III). Consequently, the Cr(VI)-reducing ability of strain CRB-1 and its chromate reductases enables CRB-1 a potential candidate for Cr(VI) bioremediation.
引用
收藏
页数:9
相关论文
共 50 条
  • [41] Rhodobacter kunshanensis sp. nov., a Novel Bacterium Isolated from Activated Sludge
    Liu, Junwei
    Bao, Yixuan
    Zhang, Xuan
    Xu, Siqiong
    Qiu, Jiguo
    He, Jian
    CURRENT MICROBIOLOGY, 2021, 78 (10) : 3791 - 3797
  • [42] Leucobacter chromiireducens CRB2, a new strain with high Cr(VI) reduction potential isolated from tannery-contaminated soil (Fez, Morocco)
    Joutey, Nezha Tahri
    Bahafid, Wifak
    Sayel, Hanane
    Nassef, Soumiya
    El Ghachtouli, Naima
    ANNALS OF MICROBIOLOGY, 2016, 66 (01) : 425 - 436
  • [43] In vitro Cr(VI) reduction by cell-free extracts of chromate-reducing bacteria isolated from tannery effluent irrigated soil
    Sumit K. Soni
    Rakshapal Singh
    Ashutosh Awasthi
    Mangal Singh
    Alok Kalra
    Environmental Science and Pollution Research, 2013, 20 : 1661 - 1674
  • [44] In vitro Cr(VI) reduction by cell-free extracts of chromate-reducing bacteria isolated from tannery effluent irrigated soil
    Soni, Sumit K.
    Singh, Rakshapal
    Awasthi, Ashutosh
    Singh, Mangal
    Kalra, Alok
    ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2013, 20 (03) : 1661 - 1674
  • [45] Exploration on the Cr(VI) resistance mechanism of a novel thermophilic Cr(VI)-reducing bacteria Anoxybacillus flavithermus ABF1 isolated from Tengchong geothermal region, China
    Yang, Wenjing
    Hong, Wanqi
    Huang, Yongji
    Li, Shuzhen
    Li, Mengke
    Zhong, Hui
    He, Zhiguo
    ENVIRONMENTAL MICROBIOLOGY REPORTS, 2022, 14 (05): : 795 - 803
  • [46] Cr(VI) reductase activity locates in the cytoplasm of Aeribacillus pallidus BK1, a novel Cr(VI)-reducing thermophile isolated from Tengchong geothermal region, China
    Ma, Yan
    Zhong, Hui
    He, Zhiguo
    CHEMICAL ENGINEERING JOURNAL, 2019, 371 : 524 - 534
  • [47] Equilibrium and Kinetic Studies on Biosorption of Cr (VI) using Novel Aspergillus Jegita isolated from Tannery Effluent
    Reya, Issac
    Prabha, Lakshmi M.
    Renitta, Emilin R.
    RESEARCH JOURNAL OF CHEMISTRY AND ENVIRONMENT, 2013, 17 (04): : 72 - 78
  • [48] Interlining Cr(VI) remediation mechanism by a novel bacterium Pseudomonas brenneri isolated from coalmine wastewater
    Banerjee, Soumya
    Kamila, Biswajit
    Barman, Sanghamitra
    Joshi, S. R.
    Mandal, Tamal
    Haider, Gopinath
    JOURNAL OF ENVIRONMENTAL MANAGEMENT, 2019, 233 : 271 - 282
  • [49] Cr(VI) reduction by Bacillus licheniformis B22 isolated from Pamukkale thermal region
    Acar, Gulumser
    Dogan, Nazime Mercan
    Evgen, Eda
    Dogan, Goksel
    CURRENT OPINION IN BIOTECHNOLOGY, 2011, 22 : S69 - S70
  • [50] Paludibacterium denitrificans sp. nov., a Novel Denitrifying Bacterium Isolated from Activated Sludge
    Ji Eun Lee
    Gyu-Min Choi
    Yong-Jae Lee
    Sang-Mi Lee
    Soon Youl Lee
    Wan-Taek Im
    Current Microbiology, 2022, 79