MicroRNA expression profiling using LNA-modified probes in a liquid-phase bead-based array

被引：9

作者：

Busch, Anna Karina ^{[1
]}

Litman, Thomas ^{[1
]}

Nielsen, Peter Stein ^{[1
]}

机构：

[1] Exiqon AS, DK-2950 Vedbaek, Denmark

来源：

NATURE METHODS | 2007年 / 4卷 / 05期

关键词：

D O I：

10.1038/nmeth1045

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we describe a new liquid-phase, bead-based array for in-solution expression analysis of microRNAs (miRNAs). The array combines Locked Nucleic Acid (LNA (TM))-modified capture probes and the xMAP (R) multiplexing bead technology. Incorporation of LNA in the array capture probes greatly increases their affinity for their short miRNA targets, thereby adding selectivity to the array. As a result, the FlexmiR (TM) system offers a highly specific, robust and fast miRNA profiling platform.

引用

页码：I / ii

页数：2

共 3 条

[1] A sensitive array for microRNA expression profiling (miChip) based on locked nucleic acids (LNA) [J].