The molecular recognition of cordycepin arabinoside and analysis of changes on cordycepin and its arabinoside in fruiting body and pupa of Cordyceps militaris

被引：5

作者：

Li, Ankang ^{[1
]}

Song, Yiqiao ^{[1
]}

Wang, Can ^{[1
]}

Wang, Jun ^{[1
]}

机构：

[1] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

来源：

FOOD CHEMISTRY | 2022年 / 389卷

关键词：

Cordyceps militaris; Cordycepin; Cordycepin arabinoside; Molecular recognition; HPLC-MS/MS; CULTURED MYCELIA; BODIES; POLYSACCHARIDES; NUCLEOSIDES; SINENSIS; GLUCOSE;

D O I：

10.1016/j.foodchem.2022.133070

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Cordyceps militaris is an edible fungus that is widely used as a functional food in many countries. In order to objectively evaluate its nutritional value, free and glycosidic cordycepins need to be analyzed. The cordycepin arabinoside molecule was recognized by the MS2 fragmentation rule, and both cordycepin and its arabinoside were quantitatively analyzed in the fruiting body and pupa of Cordyceps militaris by high-performance liquid chromatography with tandem mass spectrometric (HPLC-MS/MS). The method had good linear regression (R-2 = 0.9999), with a detection limit of 0.021 ng/mL. The recovery range was 94.32-103.09% in the fruiting body and pupa. The content of cordycepin and its arabinoside showed an upward trend with growth, and the total contents reached the highest level at the mature stage (60-70th day) without mildew. This study provides a useful reference for the evaluation and application of Cordyceps militaris as a functional food resource.

引用

页数：5

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