Carbon nanotube-chitosan system for electrochemical sensing based on dehydrogenase enzymes

被引:649
作者
Zhang, MG
Smith, A
Gorski, W [1 ]
机构
[1] Univ Texas, Dept Chem, San Antonio, TX 78249 USA
[2] Nanjing Normal Univ, Dept Chem, Nanjing 210097, Peoples R China
关键词
D O I
10.1021/ac049519u
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Muldwalled carbon nanotubes (CNT) were solubilized in aqueous solutions of a biopolymer chitosan (CHIT). The CHIT-induced solubilization of CNT facilitated their manipulations, including the modification of electrode surfaces for sensor and biosensor development. The colloidal solutions of CNT-CHIT were placed on the surface of glassy carbon (GC) electrodes to form robust CNT-CHIT films, which facilitated the electrooxidation of NADH. The GC/CNT-CHIT sensor for NADH required similar to0.3 V less overpotential than the GC electrode. The susceptibility of CHIT to chemical modifications was explored in order to covalently immobilize glucose dehydrogenase (GDH) in the CNT- CHIT films using glutaric dialdehyde (GDI). The stability and sensitivity of the GC/ CNT-CHIT-GDI-GDH biosensor allowed for the interference-free determination of glucose in the physiological matrix (urine). In pH 7.40 phosphate buffer solutions, linear least-squares calibration plots over the range 5-300 muM glucose (10 points) had slopes 80 mA M-1 cm(-2) and a correlation coefficient 0.996. The detection limit was 3 muM glucose (S/N = 3). ne CNT-CHIT system represents a simple and functional approach to the integration of dehydrogenases and electrodes, which can provide analytical access to a large group of enzymes for wide range of bioelectrochemical applications including biosensors and biofuel cells.
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收藏
页码:5045 / 5050
页数:6
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