Magnetic Glycol Chitin-Based Hydrogel Nanocomposite for Combined Thermal and D-Amino-Acid-Assisted Biofilrn Disruption

被引：37

作者：

Abenojar, Eric C. ^{[1
]}

Wickramasinghe, Sameera ^{[1
]}

Ju, Minseon ^{[1
]}

Uppaluri, Sarika ^{[1
]}

Klika, Alison ^{[2
]}

George, Jaiben ^{[2
]}

Barsoum, Wael ^{[2
]}

Frangiamore, Salvatore J. ^{[3
]}

Higuera-Rueda, Carlos A. ^{[2
]}

Samia, Anna Cristina S. ^{[1
]}

机构：

[1] Case Western Reserve Univ, Dept Chem, 10900 Euclid Ave, Cleveland, OH 44106 USA

[2] Cleveland Clin, Dept Orthopaed Surg, 9500 Euclid Ave, Cleveland, OH 44195 USA

[3] Summa Hlth Orthopaed & Sports Med, 1 Pk West Blvd, Akron, OH 44320 USA

来源：

ACS INFECTIOUS DISEASES | 2018年 / 4卷 / 08期

基金：

美国国家科学基金会;

关键词：

hydrogel nanocomposite; iron oxide nanoparticles; D-amino acids; magnetic hyperthermia; Staphylococcus aureus; biofilm; IRON-OXIDE NANOPARTICLES; RESISTANT STAPHYLOCOCCUS-AUREUS; BACTERIAL BIOFILMS; PSEUDOMONAS-AERUGINOSA; HYPERTHERMIA PROPERTIES; INFECTIONS; STRATEGIES; EFFICACY; DELIVERY; THERAPY;

D O I：

10.1021/acsinfecdis.8b00076

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Bacterial biofilms are highly antibiotic resistant microbial cell associations that lead to chronic infections. Unlike free-floating planktonic bacterial cells, the biofilms are encapsulated in a hardly penetrable extracellular polymeric matrix and, thus, demand innovative approaches for treatment. Recent advancements on the development of gel-nanocomposite systems with tailored therapeutic properties provide promising routes to develop novel antimicrobial agents that can be designed to disrupt and completely eradicate preformed biofilms. In our study, we developed a unique thermoresponsive magnetic glycol chitin-based nanocomposite containing D-amino acids and iron oxide nanoparticles, which can be delivered and undergoes transformation from a solution to a gel state at physiological temperature for sustained release of D-amino acids and magnetic field actuated thermal treatment of targeted infection sites. The D-amino acids in the hydrogel nanocomposite have been previously reported to inhibit biofilm formation and also disrupt existing biofilms. In addition, loading the hydrogel nanocomposite with magnetic nanoparticles allows for combination thermal treatment following magnetic field (magnetic hyperthermia) stimulation. Using this novel two-step approach to utilize an externally actuated gel-nanocomposite system for thermal treatment, following initial disruption with D-amino acids, we were able to demonstrate in vitro the total eradication of Staphylococcus aureus biofilms, which were resistant to conventional antibiotics and were not completely eradicated by separate D-amino acid or magnetic hyperthermia treatments.

引用

页码：1246 / 1256

页数：21