Asymmetrical labeling of giant phospholipid vesicles

被引：1

作者：

Gomiscek, G

Arrigler, V

Gros, M

Yupncic, M

Svetina, S

机构：

[1] Univ Ljubljana, Fac Med, Inst Biophys, Ljubljana 1104, Slovenia

[2] Pediat Clin, Ljubljana 1104, Slovenia

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 2000年 / 440卷 / 05期

关键词：

giant vesicles; fluorescence; asymmetrical labeling; sodium dithionite;

D O I：

10.1007/s004240000003

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. After the addition of dithionite the fluorescence on single vesicles as well as on vesicle suspension showed a 50 % decrease of its initial value corresponding to marker quenching in the outer leaflet. Hence, flourimetry as well as flourescence microscopy prove that dithionite quenching is a suitable method to induce an asymmetrical labeling of the NBD-PC marked giant vesicles.

引用

页码：R51 / R52

页数：2