Insect-resistant transgenic brinjal plants

被引：50

作者：

Kumar, PA ^{[1
]}

Mandaokar, A ^{[1
]}

Sreenivasu, K ^{[1
]}

Chakrabarti, SK ^{[1
]}

Bisaria, S ^{[1
]}

Sharma, SR ^{[1
]}

Kaur, S ^{[1
]}

Sharma, RP ^{[1
]}

机构：

[1] Indian Agr Res Inst, Natl Res Ctr Plant Biotechnol, New Delhi 110012, India

来源：

MOLECULAR BREEDING | 1998年 / 4卷 / 01期

关键词：

Bacillus thuringiensis; insecticidal crystal protein gene; crylAb; brinjal; fruit borer; Leucinodes orbonalis; transgenic plants;

D O I：

10.1023/A:1009694016179

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A synthetic cry1Ab gene coding for an insecticidal crystal protein (ICP) of Bacillus thuringiensis (Bt) was transferred to brinjal (eggplant) by cocultivating cotyledonary explants with Agrobacterium tumefaciens. Transformant plants resistant to kanamycin were regenerated. Hybridization experiments demonstrated gene integration and mRNA expression. Double-antibody sandwich ELISA analysis revealed Bt toxin protein expression in the transgenic plants. The expression resulted in a significant insecticidal activity of transgenic brinjal fruits against the larvae of fruit borer (Leucinodes orbonalis). The results also demonstrated that a synthetic gene based on monocot codon usage can be expressed in dicotyledonous plants for insect control.

引用

页码：33 / 37

页数：5

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