THE TOXICITY OF PEGAGAN (CENTELLA ASIATICA (L.) URB) FRACTIONS ON ZEBRAFISH EMBRYO

TPegagan (Centella asiatica) is an Indonesian medicinal plant with many advantages. Due to its various pharmacological effects on humans, the development of the plant as a new drug has become prominent. However, there has been no toxicity appraisal towards pegagan in fraction formulation. Thus, this research aims to determine its level of safety on zebrafish embryos. Fractionation was accomplished using the Vacuum Liquid Chromatography (VLC) method. Zebrafish embryos are divided into seven groups for each fraction, 5 groups as a various concentration and 2 groups as a control. The groups were exposed to ethyl acetate fractions in different concentrations (100 ppm; 50 ppm; 25 ppm; 12.5 ppm; 6.25 ppm). The groups were exposed to ethanol fractions in different concentrations (250 ppm; 125 ppm; 62.5 ppm; 31.25 ppm; 15.625 ppm). The positive control group was exposed to (3,4- dichloroaniline), meanwhile the negative group received dilution water. The embryos were observed every 24 hours for 96 hours using microscopes. During the study, somite abnormality, yolk-sac edema, and pericardial edema were found in zebrafish embryos. The LC50 value of the ethyl acetate fraction was 26.61 ppm, indicating a moderate level of toxicity. The LC50 value of the ethanol fraction was 808.81 ppm, categorized as low toxicity.