Evaluation of a novel system for hypothermic oxygenated pulsatile perfusion preservation

被引:18
作者
Doorschodt, Benedict M. [1 ]
Schreinemachers, Marie-Claire J. M. [2 ]
Florquin, Sandrine [3 ]
Lai, Wei [4 ]
Sitzia, Mario [4 ]
Zernecke, Alma [5 ]
Tolba, Rene H. [1 ,4 ]
机构
[1] Univ Aachen, Rhein Westfal TH Aachen, Inst Lab Anim Sci & Expt Surg, D-52074 Aachen, Germany
[2] Univ Amsterdam, Acad Med Ctr, Dept Surg, NL-1105 AZ Amsterdam, Netherlands
[3] Univ Amsterdam, Acad Med Ctr, Dept Pathol, NL-1105 AZ Amsterdam, Netherlands
[4] Univ Bonn, D-5300 Bonn, Germany
[5] Univ Aachen, Rhein Westfal TH Aachen, Inst Pathol, Inst Mol Cardiovasc Res, D-52074 Aachen, Germany
关键词
Kidney; Machine perfusion; Oxygenation; Microcirculation; Pig; BEATING DONOR KIDNEYS; MACHINE PERFUSION; COLD-STORAGE; ISCHEMIA/REPERFUSION INJURY; ORGAN PRESERVATION; LIVER PRESERVATION; GRAFT FUNCTION; TRANSPLANTATION; POLYSOL; MICROCIRCULATION;
D O I
10.1177/039139880903201004
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Background: Recently, a novel innovative machine perfusion (MP) system for hypothermic oxygenated pulsatile perfusion called the Airdrive (AD) has been developed. The aim of the study was to evaluate the biological safety of the AD system for perfusion preservation of kidney grafts in a porcine autotransplantation model using the low-viscosity perfusion solution Polysol (PS) in comparison with cold storage (CS) using PS or the University of Wisconsin solution (UW). In addition, we evaluated real-time microcirculation parameters. At sacrifice, grafts were retrieved for histological analysis and immunohistochemistry. Methods: After assessment of the microcirculation, left kidneys were retrieved. Following the washout, kidneys were preserved for 20 hr using AD-PS, CS-PS or CS-UW. Thereafter, contralateral kidneys were removed followed by heterotopic autotransplantation of the preserved graft. Seven days after transplantation animals were sacrificed with retrieval of the grafts for histological analysis. Renal function, renal microcirculation and tissue injury including the proliferative response of tubular epithelial cells (TECs) were compared. Results: Preservation using AD-PS or CS-PS resulted in higher microcirculatory flow compared with CS-UW. Improved recovery of renal function was seen in the AD-PS and CS-PS groups compared with CS-UW. Structural integrity was better preserved using AD-PS compared with both CS groups. Proliferative response of TECs was higher in CS-UW preserved grafts compared to grafts preserved using AD-PS. Conclusion: This study demonstrates the biological safety of the AD system in a porcine autotransplantation model. Also, the microcirculation was better preserved and less morphological injury was observed after 20 hr MP compared with CS. (Int J Artif Organs 2009; 32: 728-38)
引用
收藏
页码:728 / 738
页数:11
相关论文
共 50 条
[41]   A novel hypothermic machine perfusion system using a LifePort Kidney Transporter for the preservation of rat liver [J].
Zeng, Cheng ;
Hu, Xiaoyan ;
Wang, Yanfeng ;
Zeng, Xianpeng ;
Xiong, Yan ;
Li, Ling ;
Ye, Qifa .
EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2018, 15 (02) :1410-1416
[42]   Extracorporeal Hypothermic Perfusion Device for Intestinal Graft Preservation to Decrease Ischemic Injury During Transportation [J].
Munoz-Abraham, Armando Salim ;
Patron-Lozano, Roger ;
Narayan, Raja R. ;
Judeeba, Sami S. ;
Alkukhun, Abedalrazaq ;
Alfadda, Tariq I. ;
Belter, Joseph T. ;
Mulligan, David C. ;
Morotti, Raffaella ;
Zinter, Joseph P. ;
Geibel, John P. ;
Rodriguez-Davalos, Manuel I. .
JOURNAL OF GASTROINTESTINAL SURGERY, 2016, 20 (02) :313-321
[43]   Development and Characterization of a Nonelectronic Versatile Oxygenating Perfusion System for Tissue Preservation [J].
Portillo, Daniel J. ;
Gonzalez, Jose ;
Villarreal, Carorina ;
Salazar, Sophia J. ;
Fasci, Anjelyka ;
Wearden, Brandt ;
Oseghale, Jessica ;
Khalil, Anis ;
Perillo, Thomas ;
Muenchow, Leslie ;
De Lorenzo, Robert ;
Hood, R. Lyle .
ANNALS OF BIOMEDICAL ENGINEERING, 2022, 50 (08) :978-990
[44]   HYPOTHERMIC OXYGENATED PERFUSION IN LIVER TRANSPLANTATION FROM EXPANDED CRITERIA DONORS [J].
Shabunin, A. V. ;
Loran, O. B. ;
Pushkar, D. Yu. ;
Veliev, E. I. ;
Minina, M. G. ;
Drozdov, P. A. ;
Astapovich, S. A. ;
Lidzhieva, E. A. .
VESTNIK TRANSPLANTOLOGII I ISKUSSTVENNYH ORGANOV, 2024, 26 (02) :63-72
[45]   Use of the new preservation solution Custodiol-N supplemented with dextran for hypothermic machine perfusion of the kidney [J].
Gallinat, Anja ;
Lueer, Bastian ;
Swoboda, Sandra ;
Rauen, Ursula ;
Paul, Andreas ;
Minor, Thomas .
CRYOBIOLOGY, 2013, 66 (02) :131-135
[46]   Eleven hours of hypothermic oxygenated machine perfusion (HOPE) for complex liver retransplantation: A case report [J].
Boteon, Amanda P. C. S. ;
Lima, Marisa R. D. ;
Guardia, Bianca Della ;
Carvalho, Mauricio F. ;
Schlegel, Andrea ;
Boteon, Yuri L. .
ARTIFICIAL ORGANS, 2023, 47 (08) :1413-1415
[47]   Assessment of liver graft quality during hypothermic oxygenated perfusion: The first international validation study [J].
Eden, Jahnina ;
Thorne, Adam M. ;
Bodewes, Silke B. ;
Patrono, Damiano ;
Roggio, Dorotea ;
Breuer, Eva ;
Lonati, Caterina ;
Dondossola, Daniele ;
Panayotova, Guergana ;
Boteon, Amanda P. C. S. ;
Walsh, Daniel ;
Carvalho, Mauricio Flores ;
Schurink, Ivo J. ;
Ansari, Fariha ;
Kollmann, Dagmar ;
Germinario, Giuliana ;
Garrido, Elisabeth Alexis Rivas ;
Benitez, Julio ;
Rebolledo, Rolando ;
Cescon, Matteo ;
Ravaioli, Matteo ;
Berlakovich, Gabriela A. ;
De Jonge, Jeroen ;
Uluk, Deniz ;
Lurje, Isabella ;
Lurje, Georg ;
Boteon, Yuri L. ;
Guarrera, James, V ;
Romagnoli, Renato ;
Galkin, Alexander ;
Meierhofer, David ;
Porte, Robert J. ;
Clavien, Pierre Alain ;
Schlegel, Andrea ;
de Meijer, Vincent E. ;
Dutkowski, Philipp .
JOURNAL OF HEPATOLOGY, 2025, 82 (03) :523-534
[48]   Current perspectives in transplant medicine: hypothermic oxygenated perfusion [J].
Michel, Sebastian G. ;
Madsen, Joren C. .
TRANSPLANT RESEARCH AND RISK MANAGEMENT, 2016, 8 :25-30
[49]   Design and Implementation of a Hypothermic Machine Perfusion Device for Clinical Preservation of Isolated Organs [J].
Shen, Fei ;
Yan, Ruqiang .
SENSORS, 2017, 17 (06)
[50]   An Ex-Vivo Model for Hypothermic Pulsatile Perfusion of Porcine Pancreata: Hemodynamic and Morphologic Characteristics [J].
Karcz, Marcin ;
Cook, H. Terence ;
Sibbons, Paul ;
Gray, Cathy ;
Dorling, Anthony ;
Papalois, Vassilios .
EXPERIMENTAL AND CLINICAL TRANSPLANTATION, 2010, 8 (01) :55-60