Simultaneous determination of mebendazole and pyrantel pamoate from tablets by high performance liquid chromatography reverse phase (RP-HPLC)

A new, simple, precise, rapid and stability indicating RP-HPLC method has been developed for the simultaneous determination of mebendazole and pyrantel pamoate from tablets. Chromatography was carried out on a Shodex C8 column using a mixture of 0.05 M monobasic sodium phosphate: acetonitrile: triethylamine (60:40:1.5, v/v), adjusted to pH 6.8 with phosphoric acid. Detection was carried out at 290 nm using a UV detector. Retention times for pamoic acid, pyrantel base and mebendazole were 3.61, 6.41 and 12.81 min, respectively. Linearity was obtained in the concentration range of 20-160 mu g and 30-240 mu g for pyrantel pamoate and mebendazole, respectively. The method after its application for the assay of pyrantel pamoate and mebendazole from tablets, was statistically evaluated for its accuracy and precision. (C) 1997 Elsevier Science B.V.