Crystallization and preliminary X-ray analysis of the laccase from Coprinus cinereus

被引：16

作者：

Ducros, V

Davies, GJ

Lawson, DM

Wilson, KS

Brown, SH

Ostergaard, P

Pedersen, AH

Schneider, P

Yaver, DS

Brzozowski, AM

机构：

[1] JOHN INNES CTR PLANT SCI RES,NORWICH NR4 7UH,NORFOLK,ENGLAND

[2] NOVO NORDISK AS,DK-2880 BAGSVAERD,DENMARK

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1997年 / 53卷

关键词：

D O I：

10.1107/S0907444997004897

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The laccase from the fungus Coprinus cinereus has been prepared and crystallized in a form suitable for X-ray diffraction analysis. Small plate-like crystals of an enzymatically deglycosylated form of the enzyme have been grown by the hanging-drop method using polyethylene glycol as precipitant. These crystals diffract to at least 2.2 Angstrom. They belong to the space group P2(1)2(1)2(1), with cell dimensions a = 45.4, b = 85.7, c = 143.1 Angstrom with a single molecule of laccase in the asymmetric unit.

引用

页码：605 / 607

页数：3

共 21 条

[1] Structure and function of copper-containing proteins [J].