Formation of i-motif structure at neutral and slightly alkaline pH

被引:104
作者
Zhou, Jun [1 ,2 ]
Wei, Chunying [3 ]
Jia, Guoqing [1 ,2 ]
Wang, Xiuli [1 ,2 ]
Feng, Zhaochi [1 ]
Li, Can [1 ]
机构
[1] Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
[3] Shanxi Univ, Inst Mol Sci, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China
基金
中国国家自然科学基金;
关键词
C-MYC PROMOTER; NUCLEASE HYPERSENSITIVE ELEMENT; CYTOSINE-RICH STRAND; G-QUADRUPLEX; NONCONTACTING MACROMOLECULES; TETRAPLEX FORMATION; DNA NANOMACHINE; TELOMERIC MOTIF; ENERGY-TRANSFER; HUMAN GENOME;
D O I
10.1039/b919600e
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It is well known that oligonucleotides containing tracts cytosines can form i-motif structures under acidic conditions (pH < 7). However, whether i-motif can be formed under normal physiological cellular conditions (pH 7.0-7.5) is yet no conclusive proof. In the present work, using circular dichroism (CD), UV absorption spectroscopies and native polyacrylamide gel electrophoresis (PAGE), we provided the compelling evidence for the formation of i-motif structures by four cytosine clusters, [C(3)TA(2)](3)C-3 (HT), [C(4)G](3)C(4)TA (RET), C2T3C2T4C2T3C2 (CTC) and GC(2)GC(3)A(4)C(6)G (Rb), at neutral and slightly alkaline pH at 4 degrees C. Furthermore, for HT, we also supplied the evidence for the formation of i-motif structure by fluorescence resonance energy transfer (FRET) and investigated its folding kinetics. The formation time constants obtained by CD and fluorescence experiments are 214 and 493 s, respectively, indicating that HT can slowly form i-motif structure at pH 7.0 and 4 degrees C. This work implies that i-motif structures may possible form in vivo.
引用
收藏
页码:580 / 586
页数:7
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