Novel glycidyl methacrylated dextran/gelatin nanoparticles loaded with basic fibroblast growth factor: formulation and characteristics

被引:6
作者
Gu, Chunhu [1 ]
Zheng, Renhong [2 ]
Yang, Zhifu [3 ]
Wen, Aidong [3 ]
Wu, Hong [4 ]
Zhang, Hui [4 ]
Yi, Dinghua [1 ]
机构
[1] Fourth Mil Med Univ, Xijing Hosp, Dept Cardiovasc Surg, Xian 710032, Peoples R China
[2] Fourth Mil Med Univ, Xijing Hosp, Dept Hepatobiliary Surg, Xian 710032, Peoples R China
[3] Fourth Mil Med Univ, Xijing Hosp, Dept Pharm, Xian 710032, Peoples R China
[4] Fourth Mil Med Univ, Dept Pharmaceut, Xian 710032, Peoples R China
基金
中国国家自然科学基金; 国家高技术研究发展计划(863计划);
关键词
Basic fibrous growth factor; dextran; drug delivery system; gelatin; nanoparticle; CONTROLLED-RELEASE; DRUG-DELIVERY; CELL-ADHESION; DEXTRAN; GELATIN; MICROSPHERES; FIBRONECTIN; SCAFFOLDS; HYDROGELS;
D O I
10.3109/03639040902988558
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Objective: To develop a novel efficient nanoparticulate carrier loaded with basic fibroblast growth factor (bFGF). Methods: Gelatin and glycidyl methacrylate-derivatized dextran (dex-GMA) were cross-linked and polymerized to form interpenetrating polymeric networks. The properties of the nanoparticles (NPs) were investigated as a function of the degree of dex-GMA substitution and the concentration of gelatin used in the preparation of the hydrogels. The morphology was observed with scanning eletromicroscopy and transmission eletromicroscopy. The swelling, degradation, and entrapment efficiency were also determined by dynamic evaluation methods in vitro. The protein release ratio and in vitro release kinetics were evaluated by routine procedure, and the biological activity of bFGF-loaded NPs was studied by cell proliferation assay, cell attachment, and cell function. Results: The NPs have a particle size of 320 /- 20 nm. bFGF was entrapped in the nanoparticles quantitatively (the encapsulation efficiency, 89.6 /- 0.9%). The bFGF in vitro release kinetics fitted to zero-order and Higuchi equations. Proliferation assay, attachment assay, and western blot showed that bFGF NPs had good biological effects on cultured bone marrow mesenchymal stem cells and could achieve a much longer action time than bFGF solution. Conclusion: These results suggested that a novel biodegradable dex-GMA/gelatin hydrogel NPs loaded with bFGF could be successfully developed from both dextran-and gelatin-based biomaterials.
引用
收藏
页码:1419 / 1429
页数:11
相关论文
共 39 条
[1]   THE STRUCTURE OF FIBRONECTIN AND ITS ROLE IN CELLULAR ADHESION [J].
AKIYAMA, SK ;
YAMADA, KM ;
HAYASHI, M .
JOURNAL OF SUPRAMOLECULAR STRUCTURE AND CELLULAR BIOCHEMISTRY, 1981, 16 (04) :345-358
[2]   Controlled drug delivery in tissue engineering [J].
Biondi, Marco ;
Ungaro, Francesca ;
Quaglia, Fabiana ;
Netti, Paolo Antonio .
ADVANCED DRUG DELIVERY REVIEWS, 2008, 60 (02) :229-242
[3]   Release of recombinant human interleukin-2 from dextran-based hydrogels [J].
Cadée, JA ;
de Groot, CJ ;
Jiskoot, W ;
den Otter, W ;
Hennink, WE .
JOURNAL OF CONTROLLED RELEASE, 2002, 78 (1-3) :1-13
[4]   REGULATION OF PROCOLLAGEN SYNTHESIS AND PROCESSING DURING ASCORBATE-INDUCED EXTRACELLULAR-MATRIX ACCUMULATION INVITRO [J].
CHAN, D ;
LAMANDE, SR ;
COLE, WG ;
BATEMAN, JF .
BIOCHEMICAL JOURNAL, 1990, 269 (01) :175-181
[5]   In vitro evaluation of the mucoadhesive properties of polysaccharide-based nanoparticulate oral drug delivery systems [J].
Chayed, Siwar ;
Winnik, Francoise M. .
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2007, 65 (03) :363-370
[6]   Enhancement of periodontal tissue regeneration by locally controlled delivery of insulin-like growth factor-I from dextran-co-gelatin microspheres [J].
Chen, Fa-ming ;
Zhao, Yi-min ;
Wu, Hong ;
Deng, Zhi-hong ;
Wang, Qin-tao ;
Zhou, Wei ;
Liu, Qing ;
Dong, Guang-ying ;
Li, Kun ;
Wu, Zhi-fen ;
Jin, Yan .
JOURNAL OF CONTROLLED RELEASE, 2006, 114 (02) :209-222
[7]   Release of bioactive BMP from dextran-derived microspheres: A novel delivery concept [J].
Chen, FM ;
Wu, ZF ;
Sun, HH ;
Wu, H ;
Xin, SN ;
Wang, QT ;
Dong, GY ;
Ma, ZW ;
Huang, S ;
Zhang, JJ ;
Jin, Y .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2006, 307 (01) :23-32
[8]   Food oral processing - A review [J].
Chen, Jianshe .
FOOD HYDROCOLLOIDS, 2009, 23 (01) :1-25
[9]   Recent developments in cartilage research: matrix biology of the collagen II/IX/XI heterofibril network [J].
Eyre, DR ;
Wu, JJ ;
Fernandes, RJ ;
Pietka, TA ;
Weis, MA .
BIOCHEMICAL SOCIETY TRANSACTIONS, 2002, 30 :894-900
[10]   BIOLOGICAL-ACTIVITIES OF FIBROBLAST GROWTH-FACTORS [J].
GOSPODAROWICZ, D .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1991, 638 :1-8