Thymoquinone enhances the activities of enzymes related to energy metabolism in peripheral leukocytes of diabetic rats

被引：29
作者：
Fararh, Khalid M. [1 ]
Ibrahim, Adel K. [2 ]
Elsonosy, Yakot A. [3 ]
机构：
[1] Benha Univ, Dept Clin Pathol, Fac Vet Med, Toukh, Qalioubeya, Egypt
[2] Cairo Univ, Dept Clin Pathol, Fac Vet Med, Cairo, Egypt
[3] Benha Univ, Dept Biochem, Fac Vet Med, Toukh, Qalioubeya, Egypt
来源：
RESEARCH IN VETERINARY SCIENCE | 2010年 / 88卷 / 03期
关键词：
Thymoquinone; Malate dehydrogenase; Leukocytes enzymes; Energy metabolism; Diabetes mellitus; Glycemic control; MALATE-DEHYDROGENASE ACTIVITIES; NIGELLA-SATIVA; RACE HORSES; GLUCOSE; STREPTOZOTOCIN; INSULIN; DOGS; CELL; MECHANISM; MELLITUS;
D O I：
10.1016/j.rvsc.2009.10.008
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The aim of this study was to examine the effect of glycemic control using thymoquinone (TQ) on energy metabolism related enzymes in leukocytes of streptozotocin (STZ)-induced diabetic rats. The treatment of both TQ and insulin commenced 4 weeks after induction of diabetes. Plasma glucose, cholesterol and triglycerides levels were significantly reduced after TQ treatment, whereas immunoreactive insulin (IRI) showed significant increase. The activities of malate dehydrogenase (MDH) in cytosolic and mitochondrial fractions of peripheral blood leukocytes were significantly higher in rats treated with TQ and insulin as compared to that in diabetic controls. On the other hand the activities of lactic dehydrogenase (LDH) showed no significant changes between groups. ML ratio (cytosolic MDH/LDH specific activity ratio) was restored to those in the control rats. The results of this study demonstrate that TQ significantly increased insulin level and the activities of cytosolic and mitochondrial MDH in leukocytes of STZ-diabetic rats. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码：400 / 404
页数：5
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