Semiconducting Polymer Nanoreporters for Near-Infrared Chemiluminescence Imaging of Immunoactivation

被引:119
作者
Cui, Dong [1 ]
Li, Jingchao [1 ]
Zhao, Xuhui [2 ]
Pu, Kanyi [1 ]
Zhang, Ruiping [2 ]
机构
[1] Nanyang Technol Univ, Sch Chem & Biomed Engn, 70 Nanyang Dr, Singapore 637457, Singapore
[2] Shanxi Med Univ, Affiliated Da Yi Hosp, Taiyuan 030032, Shanxi, Peoples R China
基金
中国国家自然科学基金;
关键词
cancer immunotherapy; chemiluminescence imaging; organic nanoparticles; reactive oxygen species; IN-VIVO; PROBES; NANOPARTICLES; FLUORESCENT; SUPEROXIDE; ACTIVATION; TOXICITY; BIOLOGY; STRESS; CELLS;
D O I
10.1002/adma.201906314
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Real-time in vivo imaging of immunoactivation is critical for longitudinal evaluation of cancer immunotherapy, which, however, is rarely demonstrated. This study reports semiconducting polymer nanoreporters (SPNRs) with superoxide anion (O-2(center dot-))-activatable chemiluminescence signals for in vivo imaging of immunoactivation during cancer immunotherapy. SPNRs are designed to comprise an SP and a caged chemiluminescence phenoxy-dioxetane substrate, which respectively serve as the chemiluminescence acceptor and donor to enable intraparticle chemiluminescence resonance energy transfer. SPNRs are intrinsically fluorescent but only become chemiluminescent upon activation by O-2(center dot-). Representing the first O-2(center dot-)-activatable near-infrared chemiluminescent reporter, SPNR3 sensitively differentiates higher O-2(center dot-) levels in immune cells from other cells including cancer and normal cells. Following systemic administration, SPNR3 passively accumulates into tumors in living mice and activates the chemiluminescence signals responding to the concentration of O-2(center dot-) in the tumor microenvironment. Moreover, the enhancement of in vivo chemiluminescence signal after cancer immunotherapy is correlated with increased population of T cells in the tumor, proving its feasibility in tracking of T cell activation. Thus, SPNRs represent the first kind of chemiluminescent reporters competent for in vivo imaging of immunoactivation.
引用
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页数:7
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