A new method of pebrine inspection of silkworm egg using multiprimer PCR

被引：35

作者：

Hatakeyama, Y

Hayasaka, S

机构：

[1] Natl Inst Agrobiol Sci, Inst Insect & Anim Sci, Dept Insect Biotechnol & Sericol, Tsukuba, Ibaraki 3058634, Japan

[2] JST, Kawaguchi, Saitama 3320012, Japan

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 2003年 / 82卷 / 03期

关键词：

microsporidia; Nosema; bombyx mori; PCR; multiprimer; silkworm pebrine; transovum transmission;

D O I：

10.1016/S0022-2011(03)00019-3

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Using a mixture of several PCR primers, we evaluated whether multiprimer PCR is practically useful for the early and simultaneous detection of several kinds of microsporidia that cause silkworm pebrine. When genomic DNA extracted from silkworm eggs infected with Nosema bombycis was used as the DNA template, the specific DNA sequences were amplified by multiprinter PCR. In addition, similar results were obtained even when genomic DNA extracted from silkworms infected with N. bombycis was used as the DNA template. These findings suggest that multiprimer PCR using several primers designed for this study is practically useful for pebrine inspection of silkworm eggs. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：148 / 151

页数：4