Evaluation of antioxidant potential of Heliotropium bacciferum Forssk extract and wound healing activity of its topical formulation in rat

被引:3
作者
Fathalipour-Rayeni, H. [1 ]
Forootanfar, H. [2 ]
Khazaeli, P. [1 ]
Mehrabani, M. [3 ]
Rahimi, H-R [4 ]
Shakibaie, M. [5 ]
Jafari, E. [6 ]
Doostmohammadi, M. [2 ]
Bami, M. S. [1 ]
Adeli-Sardou, M. [3 ]
Shaghooei, P. M. [7 ]
Ohadi, M. [1 ]
机构
[1] Kerman Univ Med Sci, Pharmaceut Res Ctr, Inst Neuropharmacol, Kerman, Iran
[2] Kerman Univ Med Sci, Pharmaceut Sci & Cosmet Prod Res Ctr, Kerman, Iran
[3] Kerman Univ Med Sci, Herbal & Tradit Med Res Ctr, Kerman, Iran
[4] Kerman Univ Med Sci, Fac Pharm, Dept Pharmacol & Toxicol, Kerman, Iran
[5] Kerman Univ Med Sci, Fac Pharm, Dept Pharmaceut Biotechnol, Kerman, Iran
[6] Kerman Univ Med Sci, Pathol & Stem Cell Res Ctr, Pathol Dept, Kerman, Iran
[7] Shahid Bahonar Univ Kerman, Dept Biol, Kerman, Iran
来源
ANNALES PHARMACEUTIQUES FRANCAISES | 2022年 / 80卷 / 03期
关键词
Heliotropium bacciferum Forssk; Wound healing; Oxidative stress; Antioxidant; Topical formulation; ZINC NANOPARTICLES; FLAVONOIDS; AGENT;
D O I
10.1016/j.pharma.2021.09.005
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Wound healing is a dynamic process that occurs in the tissue under the skin. During this process, oxidative stress biomarkers are excessively produced, which finally lead to inflammation and cellular damage. In this study, efforts have been made to evaluate the antioxidant effect and wound healing activity topical formulation containing Heliotropium bacciferum Forssk extract. The in vitro antioxidant properties were evaluated using 2,2-diphenyl-1-picrythydrazyl (DPPH) scavenging activities and the ferric reducing antioxidant power (FRAP) assay. The herbal ointments (2.5% w/w and 5% w/w) were prepared from the hydroalcoholic extract of H. bacciferum Forssk and administrated on the induced wounds in Wistar rats. The chromatic assay, percentage of wound contraction, and histopathological studies were used for evaluating the wound heating activity. For the evaluation of reactive oxygen species (ROS), catalase (CAT) activity, superoxide dismutase (SOD), and glutathione (GSH) levels were examined. The DPPH method showed tremendous radical scavenging activities at the corresponding concentrations with EC50 value of 80 mu g/mL. Topical application of the ointment (5% w/w) showed the highest wound contraction in comparison to the positive control (treated with CICALFATE (TM)) and the control group (treated with normal saline). Similarly, the histological study of the group treated with the extract ointment (5% w/w) showed full collagen tissue deposition with a complete epidermal regeneration. The results of the assessment of GSH levels as well as CAT and SOD activities in the treated group (5% w/w) confirmed the scavenging property of the extract ointment. Our findings indicated the proper wound heating impact of the topical formulation of H. bacciferum Forssk due to its notable antioxidant capacity. (C) 2021 Academie Nationale de Pharmacie. Published by Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:280 / 290
页数:11
相关论文
共 38 条
[1]   In Vitro Antimicrobial Bioassays, DPPH Radical Scavenging Activity, and FTIR Spectroscopy Analysis of Heliotropium bacciferum [J].
Ahmad, Sohail ;
AbdEl-Salam, Naser M. ;
Ullah, Riaz .
BIOMED RESEARCH INTERNATIONAL, 2016, 2016
[2]   Heliotropium bacciferum Forssk. (Boraginaceae) extracts: chemical constituents, antioxidant activity and cytotoxic effect in human cancer cell lines [J].
Aissaoui, Hanane ;
Mencherini, Teresa ;
Esposito, Tiziana ;
De Tommasi, Nunziatina ;
Gazzerro, Patrizia ;
Benayache, Samir ;
Benayache, Fadila ;
Mekkiou, Ratiba .
NATURAL PRODUCT RESEARCH, 2019, 33 (12) :1813-1818
[3]   PHARMACOLOGICAL AND TOXICOLOGICAL EFFECTS OF HELIOTROPIUM UNDULATUM [H. BACCIFERUM] AND HELIOTROPIUM EUROPAEUM - A REVIEW [J].
Al-Snafi, Ali Esmail .
INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2018, 5 (04) :2150-2158
[4]   Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal [J].
Aryal, Sushant ;
Baniya, Manoj Kumar ;
Danekhu, Krisha ;
Kunwar, Puspa ;
Gurung, Roshani ;
Koirala, Niranjan .
PLANTS-BASEL, 2019, 8 (04)
[5]   Flavonoids in Human Health: From Structure to Biological Activity [J].
Atmani, Djebbar ;
Chaher, Nassima ;
Atmani, Dina ;
Berboucha, Meriem ;
Debbache, Nadjet ;
Boudaoud, Hania .
CURRENT NUTRITION & FOOD SCIENCE, 2009, 5 (04) :225-237
[6]   Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume [J].
Baba, Shoib A. ;
Malik, Shahid A. .
JOURNAL OF TAIBAH UNIVERSITY FOR SCIENCE, 2015, 9 (04) :449-454
[7]   Wound healing and in vitro antioxidant activities of lipopeptides mixture produced by Bacillus mojavensis A21 [J].
Ben Ayed, Hanen ;
Bardaa, Sana ;
Moalla, Dorsaf ;
Jridi, Mourad ;
Maalej, Hana ;
Sahnoun, Zouheir ;
Rebai, Tarak ;
Jacques, Phillipe ;
Nasri, Moncef ;
Hmidet, Noomen .
PROCESS BIOCHEMISTRY, 2015, 50 (06) :1023-1030
[8]  
Bhawar Sanjay B., 2019, Journal of Drug Delivery and Therapeutics, V9, P575, DOI 10.22270/jddt.v9i3-s.2917
[9]  
Dash G K, 2011, ISRN Pharmacol, V2011, P847980, DOI 10.5402/2011/847980
[10]   Assays of physical stability and antioxidant activity of a topical formulation added with different plant extracts [J].
Di Mambro, VM ;
Fonseca, MJV .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2005, 37 (02) :287-295