Outgrowing endothelial progenitor-derived cells display high sensitivity to angiogenesis modulators and delayed senescence

被引：12
作者：
Ha, Jung-Min
Kim, Myoung-Rae
Oh, Ho-Kyun
Lee, Byung Hun
Ahn, Hyun-Young
Shin, Jong-Chul
Baek, Sang Hong
Joe, Young Ae
机构：
[1] Catholic Univ Korea, Coll Med, Canc Res Inst, Seoul 137701, South Korea
[2] Catholic Univ Korea, Coll Med, Dept Biomed Sci, Seoul 137701, South Korea
[3] Catholic Univ Korea, Coll Med, Div Maternal Fetal Med, Dept Obstet & Gynecol, Seoul 137701, South Korea
[4] Catholic Univ Korea, Coll Med, Dept Internal Med, Div Cardiovasc Med, Seoul 137701, South Korea
来源：
FEBS LETTERS | 2007年 / 581卷 / 14期
关键词：
vasculogenesis; endothelial progenitor cell; senescence; angiogenesis; inhibitor;
D O I：
10.1016/j.febslet.2007.05.010
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Outgrowing endothelial progenitor-derived cells (EPDCs) originate from a novel hierarchy of endothelial progenitor cells. In this study, EPDCs isolated from human cord blood were examined for phenotype and functional features upon aging. Young or aged EPDCs were similar to human umbilical vein endothelial cells (H-UVECs), in exhibiting typical endothelial phenotypes. However, EPDCs were more sensitive to angio. genesis inducers or inhibitors in proliferation and migration. In addition, EPDCs underwent senescence markedly slowly with sustained endothelial NO synthase expression and activation, and their ability to undergo capillary morphogenesis was retained throughout longterm culture. Thus, these results suggest that a homogenous population of EPDCs derived from clonogenic expansion may provide an effective vasculogenesis tool. (c) 2007 Federation of European Biochemical Societies. Published by ElseAer B.V. All rights reserved.
引用
收藏
页码：2663 / 2669
页数：7
相关论文
共 21 条
[1]   Isolation of putative progenitor endothelial cells for angiogenesis [J].
Asahara, T ;
Murohara, T ;
Sullivan, A ;
Silver, M ;
vanderZee, R ;
Li, T ;
Witzenbichler, B ;
Schatteman, G ;
Isner, JM .
SCIENCE, 1997, 275 (5302) :964-967
[2]   Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization [J].
Asahara, T ;
Masuda, H ;
Takahashi, T ;
Kalka, C ;
Pastore, C ;
Silver, M ;
Kearne, M ;
Magner, M ;
Isner, JM .
CIRCULATION RESEARCH, 1999, 85 (03) :221-228
[3]   Transplantation of progenitor cells and regeneration enhancement in acute myocardial infarction -: (TOPCARE-AMI) [J].
Assmus, B ;
Schächinger, V ;
Teupe, C ;
Britten, M ;
Lehmann, R ;
Döbert, N ;
Grünwald, F ;
Aicher, A ;
Urbich, C ;
Martin, H ;
Hoelzer, D ;
Dimmeler, S ;
Zeiher, AM .
CIRCULATION, 2002, 106 (24) :3009-3017
[4]   The signals and pathways activating cellular senescence [J].
Ben-Porath, I ;
Weinberg, RA .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2005, 37 (05) :961-976
[5]   Human endothelial cells derived from circulating progenitors display specific functional properties compared with mature vessel wall endothelial cells [J].
Bompais, H ;
Chagraoui, J ;
Canron, X ;
Crisan, M ;
Liu, XH ;
Anjo, A ;
Port, CTL ;
Leboeuf, M ;
Charbord, P ;
Bikfalvi, A ;
Uzan, G .
BLOOD, 2004, 103 (07) :2577-2584
[6]   Premature senescence of endothelial cells: Methusaleh's dilemma [J].
Chen, J ;
Goligorsky, MS .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2006, 290 (05) :H1729-H1739
[7]   A BIOMARKER THAT IDENTIFIES SENESCENT HUMAN-CELLS IN CULTURE AND IN AGING SKIN IN-VIVO [J].
DIMRI, GP ;
LEE, XH ;
BASILE, G ;
ACOSTA, M ;
SCOTT, C ;
ROSKELLEY, C ;
MEDRANO, EE ;
LINSKENS, M ;
RUBELJ, I ;
PEREIRASMITH, O ;
PEACOCKE, M ;
CAMPISI, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (20) :9363-9367
[8]   Endothelial progenitor cell culture and differentiation in vitro: a methodological comparison using human umbilical cord blood [J].
Eggermann, J ;
Kliche, S ;
Jarmy, G ;
Hoffmann, K ;
Mayr-Beyrle, U ;
Debatin, KM ;
Waltenberger, J ;
Beltinger, C .
CARDIOVASCULAR RESEARCH, 2003, 58 (02) :478-486
[9]   Differential regulation of apoptotic cell death in senescent human cells [J].
Hampel, B ;
Malisan, F ;
Niederegger, H ;
Testi, R ;
Jansen-Dürr, P .
EXPERIMENTAL GERONTOLOGY, 2004, 39 (11-12) :1713-1721
[10]   Endothelial cellular senescence is inhibited by nitric oxide: Implications in atherosclerosis associated with menopause and diabetes [J].
Hayashi, Toshio ;
Matsui-Hirai, Hisako ;
Miyazaki-Akita, Asaka ;
Fukatsu, Akiko ;
Funami, Jun ;
Ding, Qun-Fang ;
Kamalanathan, Sumitra ;
Hattori, Yuichi ;
Ignarro, Louis J. ;
Iguchi, Akihisa .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (45) :17018-17023
123