A cosmid vector containing a dominant selectable marker for cloning Chlamydomonas genes by complementation

被引：9

作者：

Chang, M ^{[1
]}

Li, F ^{[1
]}

Odom, OW ^{[1
]}

Lee, J ^{[1
]}

Herrin, DL ^{[1
]}

机构：

[1] Univ Texas, Inst Mol & Cellular Biol, Sect Mol Cell & Dev Biol A6700, Austin, TX 78712 USA

来源：

PLASMID | 2003年 / 49卷 / 01期

关键词：

Chlamydomonas; complementation cloning; cosmid vector; dominant marker; phleomycin resistance; ble cassette;

D O I：

10.1016/S0147-619X(02)00158-0

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Cosmid vectors containing a dominate selectable marker (ble) for complementation cloning of genes in Chlamydomonas reinhardtii were created. The usefulness of these vectors, which differ in the orientation of the ble cassette, was demonstrated by transforming C reinhardtii to phleomycin resistance, by constructing a large library (5 x 10(5) recombinants) in one of them using DNA from a C reinhardtii mutant, and by transforming C reinhardtii with recombinant cosmid clones and pools. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：75 / 78

页数：4

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