Soluble or soluble/membrane TNF-α inhibitors protect the brain from focal ischemic injury in rats

被引：41

作者：

Arango-Davila, C. A. ^{[1
]}

Vera, A. ^{[2
]}

Londono, A. C. ^{[3
]}

Echeverri, A. F. ^{[4
]}

Canas, F. ^{[5
]}

Cardozo, C. F. ^{[6
]}

Orozco, J. L. ^{[7
]}

Rengifo, J. ^{[8
]}

Canas, C. A. ^{[4
]}

机构：

[1] Icesi Univ, Fdn Valle del Lili, Unit Psychiat, Cali, Colombia

[2] Caldas Univ, Sch Med, Dept Basic Sci, Manizales, Colombia

[3] Miguel Hernandez Univ, Clin Pharmacol Unit, Dept Clin Res, Hosp Gen Univ Alicante, Alicante, Spain

[4] Icesi Univ, Fdn Valle del Lili, Rheumatol Unit, Cali, Colombia

[5] CES Univ, Dept Internal Med, Fdn Valle del Lili, Cali, Colombia

[6] Icesi Univ, Fac Nat Sci, Cali, Colombia

[7] Icesi Univ, Fdn Valle del Lili, Neurol Unit, Cali, Colombia

[8] Icesi Univ, Fac Nat Sci, Cali, Colombia

来源：

INTERNATIONAL JOURNAL OF NEUROSCIENCE | 2015年 / 125卷 / 12期

关键词：

brain ischemia; anti TNF-alpha; experimental brain research; etanercept; infliximab; NECROSIS-FACTOR-ALPHA; INFLAMMATORY RESPONSE; CEREBRAL-ISCHEMIA; SERUM-LEVELS; STROKE;

D O I：

10.3109/00207454.2014.980906

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Tumor Necrosis Factor-alpha (TNF-alpha) is an immunomodulatory and proinflammatory cytokine implicated in neuro-inflammation and neuronal damage in response to cerebral ischemia. The present study tested the hypothesis that anti-TNF-alpha agents may be protective against cerebral infarction. Transient focal ischemia was artificially induced in anesthetized adult male Wistar rats (300-350 g) by middle cerebral artery occlusion (MCAO) with an intraluminal suture. TNF-alpha function was interfered with either a chimeric monoclonal antibody against TNF-alpha (infliximab-7 mg/kg) aiming to TNF-alpha soluble and membrane-attached form; or a chimeric fusion protein of TNF-alpha receptor-2 with a fragment crystallizable (Fc) region of IgG1 (etanercept-5 mg/kg) aiming for the TNF-alpha soluble form. Both agents were administered intraperitoneally 0 or 6 h after inducing ischemia. Infarct volume was measured by 2,3,5-triphenyltetrazolium chloride staining. Cerebral infarct volume was significantly reduced in either etanercept or infliximab-treated group compared with non-treated MCAO rats 24 h after reperfusion. These results suggest that anti-TNF-alpha agents may reduce focal ischemic injury in rats.

引用

页码：936 / 940

页数：5

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