Improved vectors for nisin-controlled expression in gram-positive bacteria

被引：232

作者：

Bryan, EM

Bae, T

Kleerebezem, H

Dunny, GM ^{[1
]}

机构：

[1] Univ Minnesota, Dept Microbiol, Minneapolis, MN 55455 USA

[2] Univ Minnesota, Inst Adv Studies Biol Proc Technol, Minneapolis, MN 55455 USA

[3] NIZO Food Res, Wageningen Ctr Food Sci, NL-6710 BA Ede, Netherlands

来源：

PLASMID | 2000年 / 44卷 / 02期

关键词：

D O I：

10.1006/plas.2000.1484

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A set of shuttle vectors, able to replicate in Escherichia coli and in gram-positive bacteria, containing a nisin-inducible promoter (PnisA) and genes encoding NisR and NisK, the two-component signaling mechanism for activating transcription from PnisA in the presence of nisin, was constructed. To test these vectors, Enterococcus faecalis pCF10 plasmid gents prgX, prgY, and prgZ, which respectively encode cytosolic, integral membrane, and cell surface proteins, were cloned downstream of PnisA. Increased protein expression, in the presence of nisin, was demonstrated by western blot analysis. (C) 2000 Academic Press.

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页码：183 / 190

页数：8

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