LncRNA TUG1 promoted viability and associated with gemcitabine resistant in pancreatic ductal adenocarcinoma

被引：41

作者：

Yang, Fan ^{[1
]}

Li, Xiaofang ^{[1
]}

Zhang, Lingjuan ^{[1
]}

Cheng, Lina ^{[1
]}

Li, Xiuling ^{[1
]}

机构：

[1] Zhengzhou Univ, Henan Prov Peoples Hosp, Dept Gastroenterol, Peoples Hosp, 7 Weiwu Rd, Zhengzhou 450003, Henan, Peoples R China

来源：

JOURNAL OF PHARMACOLOGICAL SCIENCES | 2018年 / 137卷 / 02期

关键词：

LncRNA TUG1; PDAC; Oncogenic; Gemcitabine resistance; LONG NONCODING RNA; CANCER; EXPRESSION; DIFFERENTIATION; PLURIPOTENCY; DIAGNOSIS; HOTAIR;

D O I：

10.1016/j.jphs.2018.06.002

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Objective: To investigate the underlying mechanism of lncRNA TUG1 in pancreatic ductal adenocarcinoma (PDAC). Methods: The expression of TUG1 was defined by qRT-PCR. The apoptotic cells were detected by flow cytometry assay. The cell migration and invasion were measured by scratch assay and Transwell assay. The level of ERK pathway was detected using Western blot. Results: Compared with normal tissues and cells, the expression of TUG1 was up-regulated in pancreatic cancer tissue and cells. Meanwhile, knockdown of TUG1 could promote PDAC cells apoptosis and inhibit PDAC cells viability, migration and invasion. In addition, overexpression of TUG1 enhanced the gemcitabine chemoresistance of PDAC cells. Surprisingly, gemcitabine combined with SCH772984 (a suppressor of ERK pathway) could reverse the drug resistance resulted from overexpression of TUG1. Conclusion: TUG1 promoted the viability of PDAC cells and enhanced its resistance of gemcitabine. (c) 2018 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society.

引用

页码：116 / 121

页数：6

共 33 条

[1] Improvements in survival and clinical benefit with gemcitabine as first-line therapy for patients with advanced pancreas cancer: A randomized trial [J].

Burris, HA ;

Moore, MJ ;

Andersen, J ;

Green, MR ;

Rothenberg, ML ;

Madiano, MR ;

Cripps, MC ;

Portenoy, RK ;

Storniolo, AM ;

Tarassoff, P ;

Nelson, R ;

Dorr, FA ;

Stephens, CD ;

VanHoff, DD .

JOURNAL OF CLINICAL ONCOLOGY, 1997, 15 (06) :2403-2413

[2] Long non-coding RNA UCA1 promotes the tumorigenesis in pancreatic cancer [J].

Chen, Ping ;

Wan, Daiwei ;

Zheng, Dingcheng ;

Zheng, Qi ;

Wu, Feng ;

Zhi, Qiaoming .

BIOMEDICINE & PHARMACOTHERAPY, 2016, 83 :1220-1226

[3] A Central Role for RAF→MEK→ERK Signaling in the Genesis of Pancreatic Ductal Adenocarcinoma [J].

Collisson, Eric A. ;

Trejo, Christy L. ;

Silva, Jillian M. ;

Gu, Shenda ;

Korkola, James E. ;

Heiser, Laura M. ;

Charles, Roch-Philippe ;

Rabinovich, Brian A. ;

Hann, Byron ;

Dankort, David ;

Spellman, Paul T. ;

Phillips, Wayne A. ;

Gray, Joe W. ;

McMahon, Martin .

CANCER DISCOVERY, 2012, 2 (08) :685-693

[4] Role of Wnt/β-catenin Signaling in Drug Resistance of Pancreatic Cancer [J].

Cui, Jiujie ;

Jiang, Weihua ;

Wang, Shuyi ;

Wang, Liwei ;

Xie, Keping .

CURRENT PHARMACEUTICAL DESIGN, 2012, 18 (17) :2464-2471

[5] Role of endoscopic ultrasound in the diagnosis of pancreatic cancer [J].

Gonzalo-Marin, Juana ;

Jose Vila, Juan ;

Perez-Miranda, Manuel .

WORLD JOURNAL OF GASTROINTESTINAL ONCOLOGY, 2014, 6 (09) :360-368

[6] Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis [J].

Gupta, Rajnish A. ;

Shah, Nilay ;

Wang, Kevin C. ;

Kim, Jeewon ;

Horlings, Hugo M. ;

Wong, David J. ;

Tsai, Miao-Chih ;

Hung, Tiffany ;

Argani, Pedram ;

Rinn, John L. ;

Wang, Yulei ;

Brzoska, Pius ;

Kong, Benjamin ;

Li, Rui ;

West, Robert B. ;

van de Vijver, Marc J. ;

Sukumar, Saraswati ;

Chang, Howard Y. .

NATURE, 2010, 464 (7291) :1071-U148

[7] lincRNAs act in the circuitry controlling pluripotency and differentiation [J].

Guttman, Mitchell ;

Donaghey, Julie ;

Carey, Bryce W. ;

Garber, Manuel ;

Grenier, Jennifer K. ;

Munson, Glen ;

Young, Geneva ;

Lucas, Anne Bergstrom ;

Ach, Robert ;

Bruhn, Laurakay ;

Yang, Xiaoping ;

Amit, Ido ;

Meissner, Alexander ;

Regev, Aviv ;

Rinn, John L. ;

Root, David E. ;

Lander, Eric S. .

NATURE, 2011, 477 (7364) :295-U60

[8] Long noncoding RNA-based chromatin control of germ cell differentiation: a yeast perspective [J].

Hiriart, Edwige ;

Verdel, Andre .

CHROMOSOME RESEARCH, 2013, 21 (6-7) :653-663

[9] The landscape of long noncoding RNAs in the human transcriptome [J].

Iyer, Matthew K. ;

Niknafs, Yashar S. ;

Malik, Rohit ;

Singhal, Udit ;

Sahu, Anirban ;

Hosono, Yasuyuki ;

Barrette, Terrence R. ;

Prensner, John R. ;

Evans, Joseph R. ;

Zhao, Shuang ;

Poliakov, Anton ;

Cao, Xuhong ;

Dhanasekaran, Saravana M. ;

Wu, Yi-Mi ;

Robinson, Dan R. ;

Beer, David G. ;

Feng, Felix Y. ;

Iyer, Hariharan K. ;

Chinnaiyan, Arul M. .

NATURE GENETICS, 2015, 47 (03) :199-+

[10] A novel long non-coding RNA-ARA: Adriamycin Resistance Associated [J].

Jiang, Min ;

Huang, Ou ;

Xie, Zuoquan ;

Wu, Shuchao ;

Zhang, Xi ;

Shen, Aijun ;

Liu, Hongchun ;

Chen, Xiaosong ;

Wu, Jiayi ;

Lou, Ying ;

Mao, Yan ;

Sun, Kan ;

Hu, Shudong ;

Geng, Meiyu ;

Shen, Kunwei .

BIOCHEMICAL PHARMACOLOGY, 2014, 87 (02) :254-283

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