A novel 1,2,4-triazole-based copper(II) complex: Synthesis, characterization, magnetic property and nuclease activity

被引：123
作者：
Li, Dong-Dong [1 ]
Tian, Jin-Lei [1 ]
Gu, Wen [1 ]
Liu, Xin [1 ]
Yan, Shi-Ping [1 ]
机构：
[1] Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
来源：
JOURNAL OF INORGANIC BIOCHEMISTRY | 2010年 / 104卷 / 02期
基金：
中国国家自然科学基金;
关键词：
1,2,4-Triazole-based complex; Magnetism; DNA cleavage; Hydrolytic cleavage; EFFICIENT HYDROLYTIC CLEAVAGE; STRAND DNA CLEAVAGE; X-RAY-STRUCTURE; CRYSTAL-STRUCTURE; METAL-COMPLEXES; PHOSPHATE DIESTER; SPECTROSCOPIC PROPERTIES; DINUCLEAR COPPER(II); BINDING; FLUORESCENCE;
D O I：
10.1016/j.jinorgbio.2009.10.020
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel binuclear copper(II) complex [Cu2L(mu-SO4)](PF6)(2) (1) (L = 3,5-bis (bis(pyridine-2-ylmethyl)amino)methyl)-4H-1,2,4-triazol-4-amine) has been synthesized and structurally characterized. X-ray structure shows that the two copper(II) atoms are bridged by one bidentate sulfate ion and the 1,2,4-triazole ring of L with Cu1 center dot center dot center dot Cu2 distance of 4.404 angstrom. Each copper(II) center has a distorted trigonal-bipyramidal configuration. Variable-temperature magnetic susceptibility studies (2-300 K) indicate the existence of weak antiferromagnetic coupling between the copper(II) ions in complex 1. The interaction of complex I with calf thymus DNA (CT-DNA) has been studied by UV absorption, fluorescence spectroscopy, circular dichroism spectroscopy, viscosity and cyclic voltammetry. Furthermore, complex 1 was able to promote single and double strand DNA cleavage in both aerobic and anaerobic conditions, the pseudo-Michaelis-Menten kinetic parameters k(cat) = 2.58 h(-1) and K-m = 1.2 x 10(-4) M were obtained for 1. The hydrolytic cleavage of DNA by the complex was supported by the evidence from free radical quenching, anaerobic experiment, thiobarbituric acid-reactive substances (TBARS) assay. (C) 2009 Elsevier Inc. All rights reserved.
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页码：171 / 179
页数：9
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