Inactivation of enveloped viruses by singlet oxygen thermally generated from a polymeric naphthalene derivative

被引:17
作者
Käsermann, F
Kempf, C
机构
[1] Univ Bern, Dept Chem & Biochem, CH-3012 Bern, Switzerland
[2] Swiss Red Cross, Blood Transfus Serv, ZLB Cent Lab, CH-3000 Bern, Switzerland
关键词
virus inactivation; singlet oxygen; Semliki Forest virus; vesicular stomatitis virus;
D O I
10.1016/S0166-3542(98)00007-2
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Inactivation of viruses can be induced by singlet oxygen generating agents. The water-insoluble polymeric compound PVNE (poly(1,4-dimethyl-6-vinylnaphthalene-1,4-endoperoxide)) is used as a storage for reactive oxygen and is able to produce thermally generated O-1(2) in a dark-reaction. Enveloped viruses from two different families, Semliki Forest virus (SFV, Togaviridae) and vesicular stomatitis virus (VSV, Rhabdoviridae) showed a loss of infectivity of up to 8 log(10)/ml (TCID50) when incubated at 37 degrees C with PVNE in buffered solutions. PVNE produces singlet oxygen by thermal decomposition without irradiation. Such chemically generated oxygen excludes reactions involving radicals (type I photoreactions), a problem often encountered in photodynamic processes utilizing dyes as sensitizers. In addition, the water insolubility of the oxygen-carrier allows an easy removal and recycling from aqueous solutions. Therefore, it may prove useful in the inactivation of viruses in biological systems and may be a helpful tool in studies concerning the inactivation mechanism by O-1(2). (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:55 / 62
页数:8
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