DNA methylation imprints on the IG-DMR of the Dlk1-Gtl2 domain in mouse male germline

被引：35
作者：
Hiura, Hitoshi
Komiyama, Junichi
Shirai, Motomu
Obata, Yayoi
Ogawa, Hidehiko
Kono, Tomohiro
机构：
[1] Tokyo Univ Agr, Dept Biosci, Setagaya Ku, Tokyo 1568502, Japan
[2] BRAIN, Tokyo 1050001, Japan
[3] Okayama Univ, Grad Sch Nat Sci & Technol, Lab Reprod Endocrinol, Okayama 7008530, Japan
来源：
FEBS LETTERS | 2007年 / 581卷 / 07期
关键词：
genomic imprinting; methylation; DMR; germ cells; mouse;
D O I：
10.1016/j.febslet.2007.02.034
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Mouse genomes show a large cluster of imprinted genes at the Dlk1-Gtl2 domain in the distal region of chromosome 12. An intergenic-differentially methylated region (IGDMR) located between Dlk1 and Gtl2 is specifically methylated in the male germline; IG-DMR regulates the parental allele-specific expression of imprinted genes. Here, we show the resetting of IG-DMR methylation marks during male germ-cell differentiation. For parental allele-specific methylation analysis, polymorphisms were detected in a 2.6-kb IG-DMR in three mouse strains. Bisulfite methylation analysis showed erasure of the marks by E14 and re-establishment before birth. The IG-DMR methylation status was maintained in spermatogonia and spermatocytes of mature testes. The IG-DMR methylation status established before birth is thus maintained throughout the lifetime in the male germline. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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收藏
页码：1255 / 1260
页数：6
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