EMDataBank unified data resource for 3DEM

被引：203

作者：

Lawson, Catherine L. ^{[1
,2
]}

Patwardhan, Ardan ^{[3
]}

Baker, Matthew L. ^{[4
]}

Hryc, Corey ^{[4
]}

Garcia, Eduardo Sanz ^{[3
]}

Hudson, Brian P. ^{[1
,2
]}

Lagerstedt, Ingvar ^{[3
]}

Ludtke, Steven J. ^{[4
]}

Pintilie, Grigore ^{[4
]}

Sala, Raul ^{[1
,2
]}

Westbrook, John D. ^{[1
,2
]}

Berman, Helen M. ^{[1
,2
]}

Kleywegt, Gerard J. ^{[3
]}

Chiu, Wah ^{[4
]}

机构：

[1] Rutgers State Univ, Dept Chem & Chem Biol, 610 Taylor Rd, Piscataway, NJ 08854 USA

[2] Rutgers State Univ, Res Collaboratory Struct Bioinformat, 610 Taylor Rd, Piscataway, NJ 08854 USA

[3] European Bioinformat Inst, European Mol Biol Lab, Prot Data Bank Europe, Wellcome Genome Campus, Cambridge CB10 1SD, England

[4] Baylor Coll Med, Verna & Marrs McLean Dept Biochem & Mol Biol, Natl Ctr Macromol Imaging, 1 Baylor Plaza, Houston, TX 77030 USA

来源：

NUCLEIC ACIDS RESEARCH | 2016年 / 44卷 / D1期

基金：

美国国家卫生研究院; 英国惠康基金; 英国医学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

PROTEIN DATA-BANK; PARTICLE ELECTRON CRYOMICROSCOPY; HIV-1 ENVELOPE GLYCOPROTEINS; VALIDATION TASK-FORCE; CRYO-EM STRUCTURE; CRYOELECTRON MICROSCOPY; ATOMIC-RESOLUTION; 3-DIMENSIONAL RECONSTRUCTION; STRUCTURAL BIOLOGY; IN-SITU;

D O I：

10.1093/nar/gkv1126

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three-dimensional Electron Microscopy (3DEM) has become a key experimental method in structural biology for a broad spectrum of biological specimens from molecules to cells. The EMDataBank project provides a unified portal for deposition, retrieval and analysis of 3DEM density maps, atomic models and associated metadata (emdatabank.org). We provide here an overview of the rapidly growing 3DEM structural data archives, which include maps in EM Data Bank and map-derived models in the Protein Data Bank. In addition, we describe progress and approaches toward development of validation protocols and methods, working with the scientific community, in order to create a validation pipeline for 3DEM data.

引用

页码：D396 / D403

页数：8

共 62 条

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