Simultaneous quantitative determination of olmesartan and hydrochlorothiazide in human plasma and urine by liquid chromatography coupled to tandem mass spectrometry

被引：38
作者：
Liu, Dongyang
Jiang, Ji
Wang, Peng
Feng, Sheng
Hu, Pei [1 ]
机构：
[1] Peking Union Med Coll Hosp, Clin Pharmacol Res Ctr, Beijing 100032, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2010年 / 878卷 / 9-10期
关键词：
Simultaneous; Determination; Olmesartan; Hydrochlorothiazide; HPLC-MS/MS; Human plasma and urine; COMBINATION; MEDOXOMIL; CS-866; PHARMACOKINETICS; THERAPY;
D O I：
10.1016/j.jchromb.2010.01.009
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A specific, sensitive and rapid method based on high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) was developed for the simultaneous determination of olmesartan (OLM) and hydrochlorothiazide (HCTZ) in human plasma and urine. Solid-phase extraction (SPE) was used to isolate the analytes from biological matrices followed by injection of the extracts onto a C-18 column with isocratic elution. Detection was carried out on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring (MRM) mode using negative electrospray ionization (ESI). The method was validated over the concentration range of 1.00-1000 ng/mL and 5.00-5000 ng/mL for OLM in human plasma and urine as well as 0.500-200 ng/mL and 25.0-25,000 ng/mL for HCTZ in human plasma and urine, respectively. Inter- and intra-run precision of OLM and HCTZ were less than 15% and the accuracy was within 85-115% for both plasma and urine. The average extraction recoveries were 96.6% and 92.7% for OLM, and 87.2% and 72.1% for HCTZ in human plasma and urine, respectively. The linearity, recovery, matrix effect and stability were validated for OLM/HCTZ in human plasma and urine. (C) 2010 Elsevier B.V. All rights reserved.
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收藏
页码：743 / 748
页数：6
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