Pedobacter miscanthi sp. nov., isolated from Miscanthus sinensis

In a survey of endophytic bacteria in Miscanthus sinensis, a strain of Gram-negative, non-endospore-forming, rod-shaped, aerobic bacterium was isolated and designated as RS10(T). Phylogenetic analysis based on its 16S rRNA gene sequence showed that the strain RS10(T) was affiliated with the genus Pedobacter and exhibited the highest sequence similarities to Pedobacter kyungheensis KACC 16221(T) (97.78 %), Pedobacter roseus KCTC 22187(T) (97.75 %), Pedobacter humicola KACC 18452(T) (97.29%) and Pedobacter soli KACC 14939(T) (97.23 %). The novel strain contained iso-C-15:0, iSO C-17:0-3OH and summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c) as major fatty acids. The only isoprenoid quinone detected in strain RS10(T) was MK-7. Strain RS10(T) contains phosphatidylethanolamine and one kind of aminophospholipid as its major polar lipids. The DNA G+C content for RS10(T) was 39.8 mol%. Based on the results of phenotypic and genomic characterizations, we concluded that strains RS10 T represents a novel species of Pedobacter, for which the name Pedobacter miscanthi sp. nov. is proposed. The type strain is RS10(T) (=KCTC 62786(T) =GDMCC 1.1415(T)).