The difference on the osteogenic differentiation between periodontal ligament stem cells and bone marrow mesenchymal stem cells under inflammatory microenviroments

被引：54
作者：
Zhang, Jing [1 ]
Li, Zhi-Gang [2 ]
Si, Ya-Meng [1 ]
Chen, Bin [1 ]
Meng, Jian [1 ]
机构：
[1] Xuzhou Cent Hosp, Xuzhou Med Coll, Affiliated Sch Clin Med, Dept Stomatol, Xuzhou, Peoples R China
[2] Xuzhou Cent Hosp, Xuzhou Med Coll, Affiliated Sch Clin Med, Dept Urol, Xuzhou, Peoples R China
来源：
DIFFERENTIATION | 2014年 / 88卷 / 4-5期
关键词：
Periodontal ligament stem cells; Bone marrow mesenchymal stem cells; Inflammation; Osteogenic differentiation; NF-KAPPA-B; TNF-ALPHA; OSTEOBLAST DIFFERENTIATION; ALKALINE-PHOSPHATASE; SIGNALING PATHWAYS; IN-VITRO; EXPRESSION; PROLIFERATION; INHIBITION; RUNX2;
D O I：
10.1016/j.diff.2014.10.001
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Periodontitis is a major cause of tooth loss in adults and periodontal ligament stem cells (PDLSCs) is the most favorable candidate for the reconstruction of tissues destroyed by periodontal diseases. However, pathological alterations caused by inflammatory insults might impact the regenerative capacities of these cells. Bone-marrow-derived human mesenchymal stem cells (11BMSC5) would accelerate alveolar bone regeneration by transplantation, compared to PDLSCs. Therefore, a better understanding of the osteogenic differentiation between PDLSCs and BMSCs in inflammatory microenviroments is therefore warranted. In this study, human PDLSCs were investigated for their stern cell characteristics via analysis of cell surface marker expression, colony forming unit efficiency, osteogenic differentiation and adipogenic differentiation, and compared to BMSCs. To determine the impact of both inflammation and the NF-kappa beta signal pathway on osteogenic differentiation, cells were challenged with TNF-alpha under osteogenic induction conditions and investigated for mineralization, alkaline phosphatase (ALP) activity, cell proliferation and relative genes expression. Results showed that PDLSCs exhibit weaker mineralization and ALP activity compared to BMSCs. TNF-alpha inhibited genes expression of osteogenic differentiation in PDLSCs, while, it stimulates gene expressions (BSP and Runx2) in BMSCs. Enhanced NF-kappa beta activity in PDLSCs decreases expression of Runx2 but it does not impede the osteogenic differentiation of BMSCs. Taken together, these results may suggest that the BMSCs owned the stronger immunomodulation in local microenvironment via anti-inflammatory functions, compared to PDLSCs. (C) 2014 International Society of Differentiation. Published by Elsevier B.V. All rights reserved,
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页码：97 / 105
页数：9
相关论文
共 67 条
[11]   Osf2/Cbfa1: A transcriptional activator of osteoblast differentiation [J].
Ducy, P ;
Zhang, R ;
Geoffroy, V ;
Ridall, AL ;
Karsenty, G .
CELL, 1997, 89 (05) :747-754
[12]   NF-κB and rel proteins:: Evolutionarily conserved mediators of immune responses [J].
Ghosh, S ;
May, MJ ;
Kopp, EB .
ANNUAL REVIEW OF IMMUNOLOGY, 1998, 16 :225-260
[13]   Expression of the osteoblast differentiation factor RUNX2 (Cbfa1/AML3/Pebp2αA) is inhibited by tumor necrosis factor-α [J].
Gilbert, L ;
He, XF ;
Farmer, P ;
Rubin, J ;
Drissi, H ;
van Wijnen, AJ ;
Lian, JB ;
Stein, GS ;
Nanes, MS .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (04) :2695-2701
[14]   TNF-α promotes fracture repair by augmenting the recruitment and differentiation of muscle-derived stromal cells [J].
Glass, Graeme E. ;
Chan, James K. ;
Freidin, Andrew ;
Feldmann, Marc ;
Horwood, Nicole J. ;
Nanchahal, Jagdeep .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (04) :1585-1590
[15]  
GOLDING B, 1988, J IMMUNOL, V141, P2564
[16]   Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo [J].
Gronthos, S ;
Mankani, M ;
Brahim, J ;
Robey, PG ;
Shi, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (25) :13625-13630
[17]   CHARACTERIZATION OF CELLS WITH OSTEOGENIC POTENTIAL FROM HUMAN MARROW [J].
HAYNESWORTH, SE ;
GOSHIMA, J ;
GOLDBERG, VM ;
CAPLAN, AI .
BONE, 1992, 13 (01) :81-88
[18]   TNFα promotes osteogenic differentiation of human mesenchymal stem cells by triggering the NF-κB signaling pathway [J].
Hess, Katrin ;
Ushmorov, Alexey ;
Fiedler, Joerg ;
Brenner, Rolf E. ;
Wirth, Thomas .
BONE, 2009, 45 (02) :367-376
[19]   Opposing TNF-α/IL-1β- and BMP-2-activated MAPK signaling pathways converge on Runx2 to regulate BMP-2-induced osteoblastic differentiation [J].
Huang, R-L ;
Yuan, Y. ;
Tu, J. ;
Zou, G-M ;
Li, Q. .
CELL DEATH & DISEASE, 2014, 5 :e1187-e1187
[20]   Differentiation of Dermal Multipotent Cells Into Odontogenic Lineage Induced by Embryonic and Neonatal Tooth Germ Cell-Conditioned Medium [J].
Huo, Na ;
Tang, Liang ;
Yang, Zhenhua ;
Qian, Hong ;
Wang, Yinxiong ;
Han, Chun ;
Gu, Zexu ;
Duan, Yinzhong ;
Jin, Yan .
STEM CELLS AND DEVELOPMENT, 2010, 19 (01) :93-103
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