Three-dimensional fluorescence excitation-emission matrix (EEM) spectroscopy with regional integration analysis for assessing waste sludge hydrolysis treated with multi-enzyme and thermophilic bacteria

被引：193
作者：
Guo, Liang [1 ,2 ]
Lu, Mingmin [2 ]
Li, Qianqian [2 ]
Zhang, Jiawen [2 ]
Zong, Yan [2 ]
She, Zonglian [2 ]
机构：
[1] Ocean Univ China, Key Lab Marine Environm & Ecol, Minist Educ, Qingdao 266100, Peoples R China
[2] Ocean Univ China, Coll Environm Sci & Engn, Qingdao 266100, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2014年 / 171卷
关键词：
Sludge hydrolysis; Multi-enzyme; Thermophilic bacteria; EEM; FRI; DISSOLVED ORGANIC-MATTER; ACTIVATED-SLUDGE; THERMOSTABLE ENZYMES; HYDROGEN-PRODUCTION; SEWAGE-SLUDGE; DIGESTION; PRETREATMENT; ENHANCEMENT; RESIDUES; MANURE;
D O I：
10.1016/j.biortech.2014.08.025
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
The hydrolysis effect of waste sludge after multi-enzyme and thermophilic bacteria pretreatments is investigated using excitation-emission matrix (EEM) with fluorescence regional integration (FRI) in this study. The compositional characteristics of extracellular polymeric substances (EPS) and dissolved organic matters (DOM) were analyzed to evaluate the sludge disintegration. The EPS and cell wall in sludge were disrupted after hydrolysis which led to carbohydrate, protein and soluble chemical oxygen demand (SCOD) of DOM increasing in sludge supernatant. The bio-degradability level in the extracted fractions of EPS and DOM depending on the fluorescence zones was found after hydrolysis. The highest proportion of percent fluorescence response (P-i,P- n) in EPS and DOM was soluble microbial by-product and humic acid-like organics. A significant increase of humic acid-like organics in DOM after thermophilic bacteria hydrolysis was obtained. The assessment of hydrolysis using EEM coupled with FRI provided a new insight toward the bio-utilization process of waste sludge. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：22 / 28
页数：7
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