False negative factor V Leiden assay following allogeneic stem cell transplant

被引：11
作者：
Crookston, KP [1 ]
Henderson, R [1 ]
Chandler, WL [1 ]
机构：
[1] Univ Washington, Dept Lab Med, Seattle, WA 98195 USA
来源：
BRITISH JOURNAL OF HAEMATOLOGY | 1998年 / 100卷 / 03期
关键词：
activated protein C resistance; factor V Leiden; thrombophilia; thrombosis; transplantation;
D O I：
10.1046/j.1365-2141.1998.00585.x
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
We report a case where a phenotypic test (an activity assay for activated protein C resistance) correctly indicated that the patient had an abnormality. whereas the initial genetic test (a PCR-based DNA assay used to detect the mutation in the FV gene) incorrectly indicated that the patient did not. The apparent false negative result of the DNA-based test was due to the use of peripheral blood leucocytes for DNA analysis. The patient had undergone a stem cell transplant several months before, and the leucocytes in her blood were derived from the stem cell donor, which lacked the FV defect.
引用
收藏
页码：600 / 602
页数：3
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