Study on bioactive PEGDA/ECM hybrid bi-layered hydrogel scaffolds fabricated by electro-writing for cartilage regeneration

被引：15

作者：

Han, Yu ^{[1
,2
]}

Lian, Meifei ^{[3
,4
]}

Zhang, Chenyu ^{[1
]}

Jia, Bo ^{[5
]}

Wu, Qiang ^{[1
]}

Sun, Benlin ^{[1
]}

Qiao, Zhiguang ^{[1
,6
]}

Sun, Binbin ^{[1
]}

Dai, Kerong ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Clin & Translat Res Ctr Printing Technol 3D, Dept Orthoped Surg,Sch Med,Shanghai Key Lab Orthop, Shanghai 200011, Peoples R China

[2] Zhengzhou Univ, Dept Orthopaed, Affiliated Hosp 1, Zhengzhou 450052, Peoples R China

[3] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Coll Stomatol, Natl Clin Res Ctr Oral Dis,Sch Med,Dept Prosthodon, Shanghai 200011, Peoples R China

[4] Shanghai Res Inst Stomatol, Shanghai 200011, Peoples R China

[5] Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Orthoped, Sch Med, Shanghai 200080, Peoples R China

[6] Shanghai Jiao Tong Univ, Renji Hosp, Dept Orthoped Surg, Sch Med, South Campus, Shanghai 201112, Peoples R China

来源：

APPLIED MATERIALS TODAY | 2022年 / 28卷

基金：

中国国家自然科学基金;

关键词：

Hybrid hydrogel bilayer scaffolds; Electro-writing; Cartilage injury; Bone marrow mesenchymal stem cells; DECELLULARIZED EXTRACELLULAR-MATRIX; MESENCHYMAL STEM-CELLS; ARTICULAR-CARTILAGE; IN-VITRO; POLY(ETHYLENE GLYCOL); HIGH-PRECISION; BONE; REPAIR; BIOINK; INTERLEUKIN-4;

D O I：

10.1016/j.apmt.2022.101547

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Articular cartilage injury is a common disease in orthopedics. Because of the poor repair ability of articular cartilage itself, medical intervention is often required for articular cartilage injury. A promising tool for cartilage injury repair is electro-writing (EW), a 3D printing technology, which can be used to prepare high-precision and high-porosity layered active scaffolds, thereby allowing it to imitate the layered structure of cartilage. In this study, we developed a hybrid ink with good EW printability and formability based on polyethylene glycol diacrylate (PEGDA) and extracellular matrix, in which cells can be mixed for printing. Moreover, growth factors were also integrated into the printed material, such that the upper layer of the scaffold was loaded with transforming growth factor-beta 1 (TGF-beta) + bone morphogenetic protein-7 (BMP-7) and the lower layer was loaded with TGF-beta+insulin growth factor-1 (IGF-1). The whole scaffold encompassed layered active ingredients and can functionally repair damaged cartilage after being implanted in the body. In summary, this study explored the printability and formability of EW hydrogel inks, based on which an original hybrid cell hydrogel ink was developed for constructing a bioactive cartilage repair scaffold capable of inducing cell differentiation to promote cartilage repair.

引用

页数：14

共 57 条

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