Role of LINC01592 in TGF-β1-induced epithelial-mesenchymal transition of retinal pigment epithelial cells

被引：0
作者：
Su, Ying [1 ]
Tang, Ziyan [1 ]
Wang, Feng [1 ]
机构：
[1] Harbin Med Univ, Dept Ophthalmol, Hosp 1, Harbin, Peoples R China
来源：
AGING-US | 2021年 / 13卷 / 10期
关键词：
long-chain non-coding RNA; retinal pigment epithelial cells; epithelial-mesenchymal transition; PROLIFERATIVE VITREORETINOPATHY; NONCODING RNAS; EXPRESSION; AUTOFLUORESCENCE; MECHANISMS;
D O I：
暂无
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Regulation of long-chain non-coding RNA01592 (LINC01592) in the process of transforming retinal pigment epithelial (RPE) cells into mesenchymal cells following induction by transforming growth factor beat 1 (TGF-beta 1) was investigated by interfering with LINC01592 expression in human RPE (hRPE) cells. LINC01592 expression in hRPE cells was significantly increased following treatment with 10 ng/mL TGF-beta 1 for 48 h. Expression of E-cadherin and Snail were decreased in hRPE cells following induction with TGF-beta 1 compared with the control group (P < 0.05). Following induction by TGF-beta 1, expression of E-cadherin, alpha-smooth muscle actin (alpha-SMA), and Snail were significantly lower in the LINC01592-knockdown group compared with the negative control group (P < 0.05). LINC01592 overexpression significantly enhanced the viability, proliferation, and migration of hRPE cells induced by TGF-beta 1 (P < 0.05). Following induction by TGF-beta 1, E-cadherin expression was significantly decreased and alpha-SMA and Snail expression were significantly increased in the LINC01592-overexpression group compared with the negative control group (P < 0.05). RPE cells induced by TGF-beta 1 exhibited epithelial-mesenchymal transition (EMT). Inhibiting LINC01592 expression could significantly reduce TGF-beta 1-induced EMT of hRPE cells. The regulatory effect of LINC01592 on EMT in hRPE cells induced by TGF-beta 1 provides a novel treatment for proliferative vitreoretinopathy.
引用
收藏
页码：14053 / 14064
页数：12
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