Therapy of mucosal candidiasis by expression of an anti-idiotype in human commensal bacteria

被引：100

作者：

Beninati, C

Oggioni, MR

Boccanera, M

Spinosa, MR

Maggi, T

Conti, S

Magliani, W

De Bernardis, F

Teti, G

Cassone, A

Pozzi, G

Polonelli, L ^{[1
]}

机构：

[1] Univ Parma, Dipartimento Patol & Med Lab, Sez Microbiol, I-43100 Parma, Italy

[2] Univ Siena, Dipartimento Biol Mol Microbiol, I-53100 Siena, Italy

[3] Univ Messina, Ist Microbiol, Messina, Italy

[4] Ist Super Sanita, Batteriol & Micol Med Lab, I-00161 Rome, Italy

来源：

NATURE BIOTECHNOLOGY | 2000年 / 18卷 / 10期

关键词：

engineered commensal bacteria; mucosal colonization; microbicidal scFv deliver; vaginal candidiasis; therapeutic treatment;

D O I：

10.1038/80250

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two recombinant strains of Streptococcus gordonii, secreting or displaying a microbicidal single-chain antibody (H6), and stably colonizing rat vagina, were used to treat an experimental vaginitis caused by Candida albicans. A post-challenge intravaginal delivery of the HG-secreting strain was as efficacious as fluconazole in rapidly abating the fungal burden. Three weeks after challenge, 75% and 37.5% of the rats treated with the He-secreting or displaying bacteria, respectively, were cured of the infection, which persisted in 100% of the animals treated with a S. gordonii strain expressing an irrelevant single-chain antibody. Thus, a human commensal bacterium can be suitably engineered to locally release a therapeutic antibody fragment.

引用

页码：1060 / 1064

页数：5

共 37 条

[31] Protegrin structure and activity against Neisseria gonorrhoeae [J].