Therapy of mucosal candidiasis by expression of an anti-idiotype in human commensal bacteria

被引：100
作者：
Beninati, C
Oggioni, MR
Boccanera, M
Spinosa, MR
Maggi, T
Conti, S
Magliani, W
De Bernardis, F
Teti, G
Cassone, A
Pozzi, G
Polonelli, L [1 ]
机构：
[1] Univ Parma, Dipartimento Patol & Med Lab, Sez Microbiol, I-43100 Parma, Italy
[2] Univ Siena, Dipartimento Biol Mol Microbiol, I-53100 Siena, Italy
[3] Univ Messina, Ist Microbiol, Messina, Italy
[4] Ist Super Sanita, Batteriol & Micol Med Lab, I-00161 Rome, Italy
来源：
NATURE BIOTECHNOLOGY | 2000年 / 18卷 / 10期
关键词：
engineered commensal bacteria; mucosal colonization; microbicidal scFv deliver; vaginal candidiasis; therapeutic treatment;
D O I：
10.1038/80250
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Two recombinant strains of Streptococcus gordonii, secreting or displaying a microbicidal single-chain antibody (H6), and stably colonizing rat vagina, were used to treat an experimental vaginitis caused by Candida albicans. A post-challenge intravaginal delivery of the HG-secreting strain was as efficacious as fluconazole in rapidly abating the fungal burden. Three weeks after challenge, 75% and 37.5% of the rats treated with the He-secreting or displaying bacteria, respectively, were cured of the infection, which persisted in 100% of the animals treated with a S. gordonii strain expressing an irrelevant single-chain antibody. Thus, a human commensal bacterium can be suitably engineered to locally release a therapeutic antibody fragment.
引用
收藏
页码：1060 / 1064
页数：5
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