L-Arabinose transport and catabolism in yeast

被引:54
作者
Fonseca, Cesar
Romao, Rute
de Sousa, Helena Rodrigues
Hahn-Hagerdal, Barbel
Spencer-Martins, Isabel [1 ]
机构
[1] Univ Nova Lisboa, Fac Sci & Technol, Biotechnol Unit, CREM, P-2829516 Caparica, Portugal
[2] Lund Univ, Dept Appl Microbiol, S-22100 Lund, Sweden
关键词
Candida arabinofermentans; L-arabinose catabolism; Pichia guilliermondii; sugar transport; yeast; SACCHAROMYCES-CEREVISIAE STRAIN; D-XYLOSE; ALDOSE REDUCTASE; FERMENTING YEAST; ETHANOLIC FERMENTATION; ALCOHOLIC FERMENTATION; HEXOSE TRANSPORTERS; GALACTOSE TRANSPORT; PENTOSE METABOLISM; ASPERGILLUS-NIGER;
D O I
10.1111/j.1742-4658.2007.05892.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two yeasts, Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012, which show rapid growth on L-arabinose and very high rates of L-arabinose uptake on screening, were selected for characterization of L-arabinose transport and the first steps of intracellular L-arabinose metabolism. The kinetics of L-arabinose uptake revealed at least two transport systems with distinct substrate affinities, specificities, functional mechanisms and regulatory properties. The L-arabinose catabolic pathway proposed for filamentous fungi also seems to operate in the yeasts studied. The kinetic parameters of the initial L-arabinose-metabolizing enzymes were determined. Reductases were found to be mostly NADPH-dependent, whereas NAD was the preferred cofactor of dehydrogenases. The differences found between the two yeasts agree with the higher efficiency of L-arabinose metabolism in C. arabinofermentans. This is the first full account of the initial steps of L-arabinose catabolism in yeast including the biochemical characterization of a specific L-arabinose transporter.
引用
收藏
页码:3589 / 3600
页数:12
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