Stimulus-evoked opioid inhibition in guinea-pig longitudinal muscle-myenteric plexus strip is modulated by NMDA receptors

被引：7

作者：

Donnerer, Josef ^{[1
]}

Liebmann, Ingrid ^{[1
]}

机构：

[1] Med Univ Graz, Inst Expt & Clin Pharmacol, A-8010 Graz, Austria

来源：

NEUROSCIENCE LETTERS | 2007年 / 419卷 / 01期

关键词：

enteric nervous system; opioid peptides; electrical stimulation; N-methyl-D-aspartate receptor; naloxone;

D O I：

10.1016/j.neulet.2007.03.053

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Longitudinal muscle-myenteric plexus (LMMP) strips of the guinea-pig ileum were used to investigate the stimulus-evoked endogenous opioid inhibition and its modulation by ionotropic glutamate receptors. Regular cholinergic twitch responses evoked by a short 3-s-field stimulation in intervals of 80 s were found reduced after an interposed period of prolonged 40-s-field stimulation. In the presence of a peptidase-inhibitor-cocktail, the cholinergic twitch response following the period of prolonged stimulation was even further reduced as compared to normal Tyrode solution without peptidase inhibitors. In both instances, the impairment of the cholinergic twitch response was completely abolished by naloxone thus demonstrating its opioidergic nature. This endogenous inhibitory opioid effect was significantly mitigated by the NMDA-receptor antagonist MK-801, but not by the AMPA/kainate receptor antagonist CNQX. These results demonstrate by functional experiments that there is a significant opioid-mediated inhibition in guinea-pig LMMP preparations evoked by a prolonged electrical stimulation, and that an NMDA antagonist can mitigate the opioid inhibition. (c) 2007 Elsevier Ireland Ltd. All rights reserved.

引用

页码：74 / 77

页数：4

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