Determining the Protein Stability of Alzheimer's Disease Protein, Amyloid Precursor Protein

被引：6

作者：

Delport, Alexandre ^{[1
]}

Hewer, Raymond ^{[1
]}

机构：

[1] Univ KwaZulu Natal, Discipline Biochem, Sch Life Sci, ZA-3201 Pietermaritzburg, South Africa

来源：

PROTEIN JOURNAL | 2019年 / 38卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

Differential scanning fluorometry; Cellular thermal shift assay; Amyloid precursor protein; Alzheimer's disease; DRUG TARGET ENGAGEMENT; THERMAL SHIFT ASSAY; LIGAND INTERACTIONS;

D O I：

10.1007/s10930-019-09829-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Determining protein thermal stability is integral in biomedical research. Here, with the use of two thermal stability assays, we show the melting temperature of amyloid precursor protein, an Alzheimer's disease related protein. The average melting temperature for amyloid precursor protein of 55.9 degrees C was derived from differential scanning fluorometry (55.1 +/- 0.3 degrees C) and cellular thermal melt (56.7 +/- 0.7 degrees C). These experimental methods have significant application for Alzheimer's disease research including their use for amyloid precursor protein stability profiling and for the identification of additional binding partners to further elucidate novel protein functions.

引用

收藏

页码：419 / 424

页数：6

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