Cytotoxicity and apoptosis induced by a new podophyllotoxin glucoside in human hepatoma (HepG2) cells

被引：0
|
作者：
Liu, Yanfeng [1 ,2 ,3 ]
Zhao, Changqi [2 ,3 ]
Li, Hongxing [2 ,3 ]
Yu, Miao [1 ,3 ]
Gao, Jiali [1 ,3 ]
Wang, Lei [4 ]
Zhai, Yonggong [1 ,2 ,3 ]
机构：
[1] Beijing Key Lab Engn Drug & Biotechnol Lab, Beijing 100875, Peoples R China
[2] Minist Educ, Key Lab Cell Proliferat & Regulat Biol, Beijing 100875, Peoples R China
[3] Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China
[4] Second Artillery Forces Gen Hosp, Dept Endocrinol, PLA, Beijing 100088, Peoples R China
来源：
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY | 2010年 / 88卷 / 04期
基金：
中国国家自然科学基金;
关键词：
podophyllotoxin; apoptosis; HepG(2); Bax; Bcl-2; CARCINOMA-CELLS; BCL-2; BAX; EXPRESSION; ETOPOSIDE;
D O I：
10.1139/Y10-008
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
4-Demethylepipodophyllotoxin 7'-O-beta-D-glucopyranoside (4' DPG). a new podophyllotoxin glucoside with a chemical structure similar to that of 4-demethylpicropodophyllotoxin 7'-O-beta-D-glucopyranoside (4DPG), was isolated from the rhizomes of Sinopodophyllum emodi (Wall.) Ying (Berberidaceae). Like 4DPG and etoposide (VP-16), 4' DPG displayed dose- and time-dependent cytotoxic effects in HepG(2) cells. Flow cytometric analysis demonstrated the presence of apoptotic cells and DNA fragmentation after HepG(2) cells were exposed to 4' DPG (1 mu mol/L). In addition, 4' DPG-driven apoptotic events were associated with upregulation of Bax and downregulation of Bcl-2. These results suggest that 4' DPG has prominent cytotoxic activity and induces apoptosis by increasing the ratio of Bax to Bcl-2 in HepG(2) cells.
引用
收藏
页码：472 / 479
页数：8
相关论文
共 50 条
  • [1] Cytotoxicity and Apoptosis Induction in Human HepG2 Hepatoma Cells by Decabromodiphenyl Ethane
    SUN Ru Bao 1
    2.Beijing Institute of Disease Control and Prevention
    BiomedicalandEnvironmentalSciences, 2012, 25 (05) : 495 - 501
  • [2] Cytotoxicity and Apoptosis Induction in Human HepG2 Hepatoma Cells by Decabromodiphenyl Ethane
    Sun Ru Bao
    Xi Zhu Ge
    Yan Jun
    Yang Hong Lian
    BIOMEDICAL AND ENVIRONMENTAL SCIENCES, 2012, 25 (05) : 495 - 501
  • [3] Capsaicin-induced Apoptosis in Human Hepatoma HepG2 Cells
    Huang, Shang-Pang
    Chen, Jung-Chou
    Wu, Chih-Chung
    Chen, Chi-Tsai
    Tang, Nou-Ying
    Ho, Yung-Tsuan
    Lo, Chyi
    Lin, Jink-Pin
    Chung, Jing-Gung
    Lin, Jaung-Geng
    ANTICANCER RESEARCH, 2009, 29 (01) : 165 - 174
  • [4] In vitro cytotoxicity and induction of apoptosis by silica nanoparticles in human HepG2 hepatoma cells
    Lu, Xun
    Qian, Jiangchao
    Zhou, Huanjun
    Gan, Qi
    Tang, Wei
    Lu, Jingxiong
    Yuan, Yuan
    Liu, Changsheng
    INTERNATIONAL JOURNAL OF NANOMEDICINE, 2011, 6 : 1889 - 1901
  • [5] Apoptosis induced by nucleosides in the human hepatoma HepG2
    Suh-Ching Yang
    Che-Lin Chiu
    Chi-Chang Huang
    Jiun-Rong Chen
    World Journal of Gastroenterology, 2005, (40) : 6381 - 6384
  • [6] Apoptosis induced by nucleosides in the human hepatoma HepG2
    Yang, Suh-Ching
    Chiu, Che-Lin
    Huang, Chi-Chang
    Chen, Jiun-Rong
    WORLD JOURNAL OF GASTROENTEROLOGY, 2005, 11 (40) : 6381 - 6384
  • [7] Size-mediated cytotoxicity and apoptosis of hydroxyapatite nanoparticles in human hepatoma HepG2 cells
    Yuan, Yuan
    Liu, Changsheng
    Qjan, Jiangchao
    Wang, Jing
    Zhang, Yuan
    BIOMATERIALS, 2010, 31 (04) : 730 - 740
  • [8] Streptozotocin-induced cytotoxicity and mitochondrial dysfunction in human hepatoma HepG2 cells
    Raza, Haider
    John, Annie
    CANCER RESEARCH, 2010, 70
  • [9] Induction of apoptosis by Xiakemycin A in human hepatoma HepG2 cells
    Chen, Chuan
    Han, Zhu
    Yang, Minjie
    Jiang, Zhongke
    Ou, Xiuyuan
    MEDICINE, 2020, 99 (17) : E19848
  • [10] Apoptosis induced by one new podophyllotoxin glucoside in human carcinoma cells
    Zhang, QY
    Jiang, M
    Zhao, CQ
    Yu, M
    Zhang, H
    Ding, YJ
    Zhai, YG
    TOXICOLOGY, 2005, 212 (01) : 46 - 53
12345