Quantifying tagged mRNA export flux via nuclear pore complexes in single live cells

被引：0
作者：
Jing, Yueyue [1 ]
Lv, Yilin [2 ,3 ]
Ye, Jingya [4 ]
Yao, Longfang [1 ]
Chen, Liwen [1 ]
Mi, Lan [1 ]
Fei, Yiyan [1 ]
Yu, Yao [2 ,3 ]
Dong, Biao [4 ]
Lv, Hong [2 ,3 ]
Ma, Jiong [1 ,5 ,6 ]
机构：
[1] Fudan Univ, Shanghai Engn Res Ctr Ultra Precis Opt Mfg, Key Lab Micro & Nano Photon Struct,Minist Educ, Dept Opt Sci & Engn,Green Photoelect Platform, Shanghai, Peoples R China
[2] Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200438, Peoples R China
[3] Shanghai Engn Res Ctr Ind Microorganisms, Shanghai 200438, Peoples R China
[4] Sichuan Univ, West China Hosp, Natl Clin Res Ctr Geriatr, Chengdu 610041, Sichuan, Peoples R China
[5] Fudan Univ, Acad Engineer & Technol, Inst Biomed Engn & Technol, Shanghai, Peoples R China
[6] Fudan Univ, Sch Life Sci, Multiscale Res Inst Complex Syst MRICS, Shanghai Engn Res Ctr Ind Microorganisms, Shanghai, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2021年 / 545卷
基金：
中国国家自然科学基金; 国家重点研发计划;
关键词：
Gene expression monitoring; RNA Transport; Nuclear pore; Fluorescence microscopy; Modeling;
D O I：
10.1016/j.bbrc.2021.01.049
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The mRNA export flux through nuclear pore complexes (NPC) changes under DNA manipulation and hence affects protein translation. However, monitoring the flux of a specific mRNA in single live cell is beyond reach of traditional techniques. We developed a fluorescence-based detection method for measuring the export flux of mRNA through NPC in single live cell using a snapshot image, which had been tested on exogenous genes' expression in HeLa cells, with transfection or infection, and endogenous genes' expression in yeast cells, during incubation and carbon catabolite repression. With its speediness, explicitness and noninvasiveness, we believe that it would be valuable in direct monitoring of gene behavior, and the understanding of gene regulation at a single cell level. (c) 2021 Elsevier Inc. All rights reserved.
引用
收藏
页码：138 / 144
页数：7
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