Determination of Meloxicam in Egg Whites and Yolks Using Reverse Phase Chromatography

被引：6

作者：

Cox, Sherry ^{[1
]}

Bailey, Joan ^{[1
]}

White, Molly ^{[1
]}

Gordon, Kristen ^{[1
]}

Souza, Marcy ^{[1
]}

机构：

[1] Univ Tennessee, Coll Vet Med, Dept Biomed & Diagnost Sci, 2407 River Dr, Knoxville, TN 37996 USA

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2017年 / 55卷 / 06期

关键词：

PERFORMANCE LIQUID-CHROMATOGRAPHY; HUMAN PLASMA; PHARMACOKINETICS;

D O I：

10.1093/chromsci/bmx014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method of analysis has been developed and validated for the determination of meloxicam in egg whites and yolks. Following a liquid extraction for the whites and a solid phase extraction for the yolks, samples were separated on an XBridge C-18 column and quantified using ultraviolet detection at 360 nm. The mobile phase was a mixture of water with glacial acetic acid and acetonitrile, with a flow rate of 1 mL/min. The procedure produced a linear graph over the concentration range 5-1500 ng/mL with a lower limit of quantification of 5 ng/mL. Intra-and inter-assay variability was 10% or less for both the whites and yolks. The average recovery for whites was 96% and the average recovery in yolks was 97%.

引用

页码：610 / 616

页数：7

共 20 条

[1]

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