Is Pomegranate juice has a vital role for protective effect on Saccharomyces cerevisiae growth?

被引:0
作者
Aslan, Abdullah [1 ]
Baspinar, Serpil [2 ]
Yilmaz, Okkes [1 ]
机构
[1] Firat Univ, Fac Sci, Dept Biol, TR-23169 Elazig, Turkey
[2] Firat Univ, Hlth Serv Vocat High Sch, TR-23169 Elazig, Turkey
来源
PROGRESS IN NUTRITION | 2014年 / 16卷 / 03期
关键词
S; cerevisiae; Pomegranate juice; oxidative damage; SDS-PAGE; POLYUNSATURATED FATTY-ACIDS; PROTEINS; CELLS; H2O2;
D O I
暂无
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
In this study, four groups were composed. i: Control group, ii: H2O2 group, iii: Pomegranate juice (PJ) group and iv: PJ + H2O2 group. After sterilization, pj (20%) and H2O2 (6% v/v) were inserted to Saccharomyces cerevisiae (S. cerevisiae) cultures and the cultures were developed at 60 degrees C for 1h, 2h, 4h and 72 hours (overnight). Fatty acids and vitamin values were gauged by HPLC and GC, the total protein was determined by SDS- PAGE. with respect to our studies results; fatty acid synthesis rised in groups to which PJ was taken in proportion to the control group (p< 0,05). Vitamin ingredient also rised particularly in the group to which PJ was taken in proportion to the control (p< 0,05). In addition, cell intensity was gauged to be upward of in PJ groups in proportion to the control (p< 0,05), whereas banding was observed more in PJ groups in proportion to the control. As a result PJ has a protective role for decrease the oxidative damage in S. cerevisiae.
引用
收藏
页码:212 / 217
页数:6
相关论文
共 28 条
[1]   Copper ions mediate the lethality induced by hydrogen peroxide in low iron conditions in Escherichia coli [J].
Almeida, CEB ;
Galhardo, RS ;
Felício, DL ;
Cabral-Neto, JB ;
Leitao, AC .
MUTATION RESEARCH-DNA REPAIR, 2000, 460 (01) :61-67
[2]  
Bienert GP, 2013, BIOCHIM BIOPHYS ACTA, V1838, P216
[3]   Protein damage, repair and proteolysis [J].
Chondrogianni, Niki ;
Petropoulos, Isabelle ;
Grimm, Stefanie ;
Georgila, Konstantina ;
Catalgol, Betul ;
Friguet, Bertrand ;
Grune, Tilman ;
Gonos, Efstathios S. .
MOLECULAR ASPECTS OF MEDICINE, 2014, 35 :1-71
[4]   Saccharomyces cerevisiae strain expressing a plant fatty acid desaturase produces polyunsaturated fatty acids and is susceptible to oxidative stress induced by lipid peroxidation [J].
Cipak, A ;
Hasslacher, M ;
Tehlivets, O ;
Collinson, EJ ;
Zivkovic, M ;
Matijevic, T ;
Wonisch, W ;
Waeg, G ;
Dawes, IW ;
Zarkovic, N ;
Kohlwein, SD .
FREE RADICAL BIOLOGY AND MEDICINE, 2006, 40 (05) :897-906
[5]   Selected non-Saccharomyces wine yeasts in controlled multistarter fermentations with Saccharomyces cerevisiae [J].
Comitini, Francesca ;
Gobbi, Mirko ;
Domizio, Paola ;
Romani, Cristina ;
Lencioni, Livio ;
Mannazzu, Ilaria ;
Ciani, Maurizio .
FOOD MICROBIOLOGY, 2011, 28 (05) :873-882
[6]  
Costa Vitor, 2001, Molecular Aspects of Medicine, V22, P217, DOI 10.1016/S0098-2997(01)00012-7
[7]   Hydrogen peroxide-induced carbonylation of key metabolic enzymes in Saccharomyces cerevisiae:: The involvement of the oxidative stress response regulators Yap1 and Skn7 [J].
Costa, WMV ;
Amorim, MA ;
Quintanilha, A ;
Moradas-Ferreira, P .
FREE RADICAL BIOLOGY AND MEDICINE, 2002, 33 (11) :1507-1515
[8]  
Dilsiz N, 1997, CELL BIOCHEM FUNCT, V15, P265, DOI 10.1002/(SICI)1099-0844(199712)15:4<265::AID-CBF750>3.0.CO
[9]  
2-D
[10]   H2O2 induces rapid biophysical and permeability changes in the plasma membrane of Saccharomyces cerevisiae [J].
Folmer, Vanderlei ;
Pedroso, Nuno ;
Matias, Ana C. ;
Lopes, Silvia C. D. N. ;
Antunes, Fernando ;
Cyrne, Luisa ;
Marinho, H. Susana .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2008, 1778 (04) :1141-1147